Vinegar And Citric Acid(Biotechnology ) Questions and Answers

Question 1. Vinegar is typically produced in fed batch reactors because
  1.    a fed batch reactor can be used to maintain low acetic acid concentrations
  2.    a fed batch reactor can be used to maintain low ethanol concentrations
  3.    All of these
  4.    acetic acid bacteria tend to ferment at high ethanol concentrations
Explanation:-
Answer: Option B. -> a fed batch reactor can be used to maintain low ethanol concentrations

Question 2. Spirit vinegar is produced from
  1.    ale
  2.    ethanol
  3.    fruit juices
  4.    malted grain
Explanation:-
Answer: Option B. -> ethanol

Question 3. For the recovery of citric acid after fermentation, Ca(OH)2 is added to the slurry to
  1.    precipitate calcium phosphate
  2.    precipitate calcium carbonate
  3.    precipitate calcium citrate
  4.    precipitate calcium sulphate
Explanation:-
Answer: Option C. -> precipitate calcium citrate

Question 4. Sugar content of the fermentation medium for citric acid is maintained at
  1.    10-15%
  2.    15-20%
  3.    25-30 %
  4.    20-25 %
Explanation:-
Answer: Option D. -> 20-25 %

Question 5. Which of the following microorganism is used for the production of citric acid?
  1.    Lactobacillus bulgaricus
  2.    Saccharomyces cerevisiae
  3.    Aspergillus niger
  4.    Streptococcus lactis
Explanation:-
Answer: Option C. -> Aspergillus niger

Question 6. What is the pH of the medium when sucrose is used as substrate for the production of citric acid?
  1.    4
  2.    6
  3.    3
  4.    5
Explanation:-
Answer: Option C. -> 3

Question 7. In koji process for the fermentation to produce citric acid,
  1.    both (a) and (b)
  2.    Fungal mycelium grows through out the solution in a deep tank
  3.    A. niger floats on the surface of solution
  4.    A. niger is used with solid substrate as sweet potato
Explanation:-
Answer: Option D. -> A. niger is used with solid substrate as sweet potato

Question 8. What is the pH of the medium when molasses is used as substrate for the production of citric acid?
  1.    4
  2.    3
  3.    6
  4.    5
Explanation:-
Answer: Option D. -> 5

Question 9. Which of the following nitrogen sources is added in the fermentation medium of citric acid production?
  1.    Urea
  2.    Both (a) and (b)
  3.    Ammonium salt
  4.    Peptone
Explanation:-
Answer: Option B. -> Both (a) and (b)

Question 10. In liquid surface culture process for the fermentation to produce citric acid,
  1.    A. niger is used with solid substrate as sweet potato
  2.    A. niger floats on the surface of solution
  3.    both (a) and (b)
  4.    Fungal mycelium grows through out the solution in a deep tank
Explanation:-
Answer: Option B. -> A. niger floats on the surface of solution