Discussion Forum : Genomic And Screening Libraries
Question - If hybridization is carried out with a clone having a sequence similar to that of the vector from which library has been constructed, then it is of no use. Choose the correct statement in respect to it.
Options:
A .  PCR amplification of the probe can be carried out
B .  Excision of the probe should be carried out which should be followed polyacrylamide gel electrophoresis
C .  If the vector sequence and the DNA probe are having similarities, some of the vector would be hybridized
D .  Probe should be in a vector that is having some sequence similarity with the library to be screened
Answer: Option A
Answer: (a).PCR amplification of the probe can be carried out
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