Discussion Forum : Genomic And Screening Libraries
Question - The screening of libraries us carried out by nucleic acid hybridisation and constitutes of following steps:
i) Peeling of membranes carrying away bacterial cells with it
ii) Cells are lysed and denaturing of DNA is being carried out
iii) Hybridization with labelled DNA
iv) Placing the membrane onto plate containing recombinant cells
Choose the correct sequence in which the steps are carried out (starting to ending).
Options:
A .  iv)-i)-ii)-iii)
B .  iv)-ii)-i)-iii)
C .  i)-ii)-iii)-iv)
D .  iii)-ii)-i)-iv)
Answer: Option A
Answer: (a).iv)-i)-ii)-iii)

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