Vectors Uses For Animal Cell Culture(Biotechnology ) Questions and Answers

Question 1.  Yeast Replicating plasmids (YRp) carry
  1.    centromere
  2.    telomere
  3.    autonomously replicating sequence
  4.    both (a) and (c)
Explanation:-
Answer: Option C. -> autonomously replicating sequence
Question 2.  Which of the following viruses are used as vectors for transferring genes to animal cells?
  1.    SV 40 virus
  2.    Bovine papilloma virus (BVP)
  3.    Retroviruses
  4.    All of these
Explanation:-
Answer: Option D. -> All of these
Question 3.  Recombinant adeno virus can be created by deleting E1A/E 1B genes that are
  1.    transcriptional regulators and are essential for viral replication
  2.    not necessary for viral replication
  3.    coding for virus coat protein
  4.    important for packaging of viral DNA into viral head
Explanation:-
Answer: Option A. -> transcriptional regulators and are essential for viral replication
Question 4.  Using bovine papilloma virus (BPV) derived vectors, permanent cell lines can be obtained carrying a recombinant DNA
  1.    episomally only
  2.    integrated only
  3.    either episomally or integrated at high copy number
  4.    either episomally or integrated at low copy number
Explanation:-
Answer: Option C. -> either episomally or integrated at high copy number
Question 5.  Which of the following histone is not present in SV40 virus?
  1.    H1
  2.    H2A
  3.    H3
  4.    H4
Explanation:-
Answer: Option A. -> H1
Question 6.  Retroviruses have advantage for being used as vector for animal cells because
  1.    they cover a wide host range
  2.    infection does not lead to cell death, infected cells produce virus over an indefinite period
  3.    viral gene expression is driven by strong promoters
  4.    all of the above
Explanation:-
Answer: Option D. -> all of the above
Question 7.  Autonomously replicating sequence is not present in
  1.    YIp
  2.    YRp
  3.    YCp
  4.    YAC
Explanation:-
Answer: Option A. -> YIp
Question 8.  In case of Baculoviruses, the foreign gene
  1.    is inserted downstream of polyhedrin promoter
  2.    is inserted downstream of viral origin of replication
  3.    is inserted between the polyhedrin promoter and downstream sequences
  4.    is inserted randomly in viral genome
Explanation:-
Answer: Option C. -> is inserted between the polyhedrin promoter and downstream sequences
Question 9.  α-aminoadipate inhibits the growth if one of the following marker is present in the yeast. That is
  1.    His 3
  2.    Lys 2
  3.    Ura 3
  4.    Trp 1
Explanation:-
Answer: Option B. -> Lys 2
Question 10. . Yeast episomal plasmids (YEp) were constructed using
  1.    2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pMB 9
  2.    2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pBR 322
  3.    12μm yeast plasmid and E coli vector pMB 9
  4.    12μm yeast plasmid and E coli vector pBR 322
Explanation:-
Answer: Option A. -> 2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pMB 9
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