Spectroscopy(Biochemistry ) Questions and Answers

Question 1.  Molar absorbtivities of compounds exhibiting charge transfer absorption are
  1.    small
  2.    moderate
  3.    large
  4.    none of these
Explanation:-
Answer: Option C. -> large
Question 2.  Which of the following relationships between absorbance and %transmittance is incorrect?
  1.    A = log10 100 / %T
  2.    A = 2 - log10 %T
  3.    A = log10 1 / T
  4.    All are correct
Explanation:-
Answer: Option C. -> A = log10 1 / T
Question 3.  In the past, IR spectra had to be aquired one wavelength at a time, which took a long time. Today quick spectra is due to the
  1.    the Fourier Transfer Algorithm allows us to scan all frequencies at once
  2.    light is faster today that it used to be
  3.    absence of broad spectrum of wavelenth
  4.    none of the above
Explanation:-
Answer: Option A. -> the Fourier Transfer Algorithm allows us to scan all frequencies at once
Question 4.  Molar absorbtivity is the measure of the
  1.    amount of light absorbed per unit length
  2.    amount of light absorbed per unit concentration
  3.    amount of light reflected and absorbed per unit concentration
  4.    None of the above
Explanation:-
Answer: Option B. -> amount of light absorbed per unit concentration
Question 5.  Which of the following is not an IR vibrational mode?
  1.    Stretching
  2.    Scissoring
  3.    Rocking
  4.    Rolling
Explanation:-
Answer: Option D. -> Rolling
Question 6.  Why is it generally preferable to use absorbance as a measure of absorption rather than % transmittance?
  1.    Because %T cannot be measured as accurately as absorbance
  2.    Because %T is dependant on the power of the incident radiation
  3.    Because absorbance is proportional to the concentration of the analyte, whereas %T is not
  4.    none of the above
Explanation:-
Answer: Option C. -> Because absorbance is proportional to the concentration of the analyte, whereas %T is not
Question 7.  Which of the following will oscillate the fastest?
  1.    A large mass on a weak spring
  2.    A large mass on a stiff spring
  3.    A small mass on a stiff spring
  4.    A small mass on a weak spring
Explanation:-
Answer: Option C. -> A small mass on a stiff spring
Question 8.  In the equation, A = ε bc, what quantity is represented by "ε"?
  1.    Absorbtivity
  2.    Molar absorbtivity
  3.    Path length
  4.    None of these
Explanation:-
Answer: Option B. -> Molar absorbtivity
Question 9.  Why must the voltage supplied to a tungsten lamp be very stable?
  1.    Because if it wasn't, the lamp would bum out
  2.    Because amount of energy the lamp emits is proportional to the fourth power of the operating voltage
  3.    Because the lamp will only function at a specific voltage
  4.    All of the above
Explanation:-
Answer: Option B. -> Because amount of energy the lamp emits is proportional to the fourth power of the operating voltage
Question 10. . Where does a carbonyl (C=0) stretch appear in an IR spectrum?
  1.    1740-1720
  2.    1870-1650
  3.    3640-3250
  4.    160-110
Explanation:-
Answer: Option B. -> 1870-1650
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