Chromatography(Biochemistry ) Questions and Answers

Question 1.  Thin layer chromatography is
  1.    partition chromatography
  2.    electrical mobility of ionic species
  3.    adsorption chromatography
  4.    none of the above
Explanation:-
Answer: Option C. -> adsorption chromatography
Question 2.  Ion exchange chromatography is based on the
  1.    electrostatic attraction
  2.    electrical mobility of ionic species
  3.    adsorption chromatography
  4.    partition chromatography
Explanation:-
Answer: Option A. -> electrostatic attraction
Question 3.  In reverse phase chromatography, the stationary phase is made
  1.    non-polar
  2.    polar
  3.    either non-polar or polar
  4.    none of these
Explanation:-
Answer: Option A. -> non-polar
Question 4.  In gas chromatography, the basis for separation of the components of the volatile material is the difference in
  1.    partition coefficients
  2.    conductivity
  3.    molecular weight
  4.    molarity
Explanation:-
Answer: Option A. -> partition coefficients
Question 5.  A combination of paper chromatography and electrophoresis involves
  1.    partition chromatography
  2.    electrical mobility of the ionic species
  3.    both (a) and (b)
  4.    none of these
Explanation:-
Answer: Option C. -> both (a) and (b)
Question 6.  The general expression for the appearance of a solute in an effluent is (where V is the elution volume of a substance , V0 void volume, kD distribution constant and Vi internal water volume)
  1.    V = V0 + kDVi
  2.    V = V0/Vi
  3.    V = V0 - kDVi
  4.    V/V0 = kDVi
Explanation:-
Answer: Option A. -> V = V0 + kDVi
Question 7.  Thin layer chromatography is
  1.    partition chromatography
  2.    electrical mobility of ionic species
  3.    adsorption chromatography
  4.    none of the above
Explanation:-
Answer: Option C. -> adsorption chromatography
Question 8.  In gas chromatography, the basis for separation of the components of the volatile material is the difference in
  1.    partition coefficients
  2.    conductivity
  3.    molecular weight
  4.    molarity
Explanation:-
Answer: Option A. -> partition coefficients
Question 9. . In reverse phase chromatography, the stationary phase is made
  1.    non-polar
  2.    polar
  3.    either non-polar or polar
  4.    none of these
Explanation:-
Answer: Option A. -> non-polar
Question 10. . A combination of paper chromatography and electrophoresis involves
  1.    partition chromatography
  2.    electrical mobility of the ionic species
  3.    both (a) and (b)
  4.    none of these
Explanation:-
Answer: Option C. -> both (a) and (b)
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