Ft Ir Spectroscopy(Biochemistry ) Questions and Answers

Question 1.  Why is it advantageous to record many FID signals from the same sample and then add them together?
  1.    To ensure that all target nuclei in the sample have been excited
  2.    To remove inaccuracies caused by fluctuations in the applied magnetic field
  3.    To increase sensitivity
  4.    None of the above
Explanation:-
Answer: Option C. -> To increase sensitivity
Question 2.  What is the name given to the relaxation process due to an interaction between an excited nucleus and the magnetic fields caused by nuclei in molecules moving around in the sample?
  1.    Spin - lattice relaxation
  2.    Spin - spin relaxation
  3.    Spin - spin - spin relaxation
  4.    None of these
Explanation:-
Answer: Option A. -> Spin - lattice relaxation
Question 3.  What is meant by rotating frame of reference?
  1.    That the sample is spun rapidly in the applied field
  2.    If the laboratory itself is imagined to be rotated at the Larmor frequency, viewing that individual magnetic moment vectors are fixed in space
  3.    That the detector rotates around the sample
  4.    None of the above
Explanation:-
Answer: Option B. -> If the laboratory itself is imagined to be rotated at the Larmor frequency, viewing that individual magnetic moment vectors are fixed in space
Question 4.  Cytochrome C has an isoelectric pH of
  1.    8.5
  2.    10.05
  3.    7.5
  4.    11.05
Explanation:-
Answer: Option B. -> 10.05
Question 5.  Which of the following is the basis of first dimension of separation for two-dimensional electrophoresis?
  1.    Molecular mass
  2.    Solubility
  3.    Isoelectric point
  4.    Folding
Explanation:-
Answer: Option C. -> Isoelectric point
Question 6.  
The sequence of amino acids in proteins can be determined by means of
  1.    identification of the -NH2 terminal amino acids
  2.    identification of the -COOH terminal amino acids
  3.    partial cleavage of the original polypeptide into smaller polypeptides
  4.    all of the above
Explanation:-
Answer: Option D. -> all of the above
Question 7.  In FT-NMR, how are nuclei excited?
  1.    By radio-frequency radiation whose frequency is swept across a predetermined range
  2.    By an intense pulse of radiation which contains a wide range of frequencies
  3.    By an intense pressure
  4.    None of the above
Explanation:-
Answer: Option A. -> By radio-frequency radiation whose frequency is swept across a predetermined range
Question 8.  What does the Michelson interferometer do?
  1.    Split a polychromatic beam of radiation into its component wavelengths
  2.    Selectively filter certain wavelengths from a beam of I.R. radiation
  3.    Modulate the I.R. signal at a lower frequency, so that it can be observed by a detector
  4.    none of the above
Explanation:-
Answer: Option C. -> Modulate the I.R. signal at a lower frequency, so that it can be observed by a detector
Question 9.  In scanning electron microscopy
  1.    a specimen is fixed and then coated with thin layer of a heavy metal
  2.    a specimen is fixed and then coated with transition metal
  3.    a specimen is not fixed and then coated with thin layer of a heavy metal
  4.    none of the above
Explanation:-
Answer: Option A. -> a specimen is fixed and then coated with thin layer of a heavy metal
Question 10. .  How many possible orientations do spin 1/2 nuclei have when they are located in an applied magnetic field?
  1.    2
  2.    4
  3.    3
  4.    6
Explanation:-
Answer: Option A. -> 2
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