Protein And Nucleic Acid Interactions(Biochemistry ) Questions and Answers

Question 1.  Reverse transcripase from HIV does not
  1.    uses RNA as a template
  2.    has a 5' to 3' polymerase activity
  3.    has a 3'- 5' exonuclease activity
  4.    requires a primer
Explanation:-
Answer: Option C. -> has a 3'- 5' exonuclease activity
Question 2.  The enzyme that joins DNA cuts is called
  1.    joinase
  2.    ligase
  3.    DNA phosphorylase
  4.    reverse transcriptase
Explanation:-
Answer: Option B. -> ligase
Question 3.  The type II restriction endonucleases, bind to DNA sites
  1.    using H-bonds for specificity
  2.    that are usually palindromic
  3.    using non-sequence-dependent backbone interactions
  4.    all of the above
Explanation:-
Answer: Option D. -> all of the above
Question 4.  Which of the following amino acids can interact with the DNA or RNA nucleotide bases via hydrogen bonding?
  1.    Asn and Gln
  2.    Cys and Met
  3.    Lys and Leu
  4.    Ile and Val
Explanation:-
Answer: Option A. -> Asn and Gln
Question 5.  Different DNA polymerases play distinct roles in DNA replication and repair in both prokaryotic and eukaryotic cells. All known DNA polymerases synthesize DNA only in the __________ by the addition of dNTPs to a performed primer strand of DNA.
  1.    positive direction
  2.    3' to 5' direction
  3.    5' to 3' direction
  4.    negative direction
Explanation:-
Answer: Option C. -> 5' to 3' direction
Question 6.  Which of the following amino acids interact with the DNA or RNA backbone (i.e. phosphate)?
  1.    Ile and Val
  2.    Lys and Arg
  3.    Leu and Ala
  4.    Cys and Met
Explanation:-
Answer: Option B. -> Lys and Arg
Question 7.  Cleavage of DNA by EcoRI endonuclease results in the formation of
  1.    two 3'-OH ends and two 5'-phosphate ends
  2.    one 3'-OH end and one 5'-phosphate end
  3.    two 3'-phosphate ends and two 3'-OH ends
  4.    one 3'-OH end and two 5'-phosphate end
Explanation:-
Answer: Option A. -> two 3'-OH ends and two 5'-phosphate ends
Question 8.  Reverse transcripase from HIV does not
  1.    uses RNA as a template
  2.    has a 5' to 3' polymerase activity
  3.    has a 3'- 5' exonuclease activity
  4.    requires a primer
Explanation:-
Answer: Option C. -> has a 3'- 5' exonuclease activity
Question 9. . Different DNA polymerases play distinct roles in DNA replication and repair in both prokaryotic and eukaryotic cells. All known DNA polymerases synthesize DNA only in the __________ by the addition of dNTPs to a performed primer strand of DNA.
  1.    positive direction
  2.    3' to 5' direction
  3.    5' to 3' direction
  4.    negative direction
Explanation:-
Answer: Option C. -> 5' to 3' direction
Question 10. . The type II restriction endonucleases, bind to DNA sites
  1.    using H-bonds for specificity
  2.    that are usually palindromic
  3.    using non-sequence-dependent backbone interactions
  4.    all of the above
Explanation:-
Answer: Option D. -> all of the above
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