Transcription(Microbiology ) Questions and Answers

Question 1. Which of the following statement defines a replicon?
  1.    A DNA template which is used in transcription
  2.    A DNA molecule that is able to replicate and be maintained
  3.    A DNA molecule that encodes pili for conjugation
  4.    The enzyme responsible for transposition
Explanation:-
Answer: Option B. -> A DNA molecule that is able to replicate and be maintained
Question 2. What is the usual sequence of a Pribnow box?
  1.    UUUUU
  2.    AUAUA
  3.    TTGACA
  4.    TATAAT
Explanation:-
Answer: Option D. -> TATAAT
Question 3. Which of the following enzyme is used for synthesis of RNA under the direction of DNA?
  1.    DNA polymerase
  2.    RNA polymerase
  3.    DNA ligase
  4.    RNA ligase
Explanation:-
Answer: Option B. -> RNA polymerase
Question 4. Which of the following component of RNA polymerase facilitates the recognition of promoter sequences?
  1.    s subunit
  2.    c subunit
  3.    b subunit
  4.    a subunit
Explanation:-
Answer: Option A. -> s subunit
Question 5. Which eukaryotic RNA polymerase makes tRNA's?
  1.    RNA polymerase 2
  2.    RNA polymerase 1
  3.    RNA polymerase 3
  4.    Any of these
Explanation:-
Answer: Option C. -> RNA polymerase 3
Question 6. The location of first hexameric sequence typically found in pro-karyotic promoters is
  1.    approximately 35 bases upstream of the transcription start site
  2.    at the transcription start site
  3.    approximately 10 bases upstream of the transcription start site
  4.    approximately 25 bases upstream of the transcription start site
Explanation:-
Answer: Option A. -> approximately 35 bases upstream of the transcription start site
Question 7. Which of the following is a product of transcription?
  1.    mRNA
  2.    tRNA
  3.    all of these
  4.    rRNA
Explanation:-
Answer: Option C. -> all of these
Question 8. The synthesis of mRNA based on a DNA template is called
  1.    translation
  2.    DNA replication
  3.    DNA restriction
  4.    transcription
Explanation:-
Answer: Option D. -> transcription
Question 9. The sigma factor
  1.    provides the catalytic function of cleaving off pyrophosphates during mRNA synthesis
  2.    recognizes the promotor
  3.    accomplishes DNA restriction
  4.    is responsible for recognizing the termination sequence
Explanation:-
Answer: Option B. -> recognizes the promotor
Question 10. Recognition/binding site of RNA polymerase is called
  1.    terminator
  2.    promoter
  3.    facilitator
  4.    receptor
Explanation:-
Answer: Option B. -> promoter
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