Identification Of Bacteria(Microbiology ) Questions and Answers

Question 1. The gram-negative organisms is
  1.    Clostridium
  2.    Bacillus
  3.    Actinomyces
  4.    None of these
Explanation:-
Answer: Option D. -> None of these
Question 2. Which of the following is a substitute for crystal violet used in gram-staining procedure?
  1.    Phenolpthalene
  2.    Methylene blue
  3.    Safranin
  4.    Bromocresol green
Explanation:-
Answer: Option B. -> Methylene blue
Question 3. Catalase production is negative in which of the following?
  1.    Staphylococcus
  2.    Streptococcus
  3.    Salmonella
  4.    Proteus
Explanation:-
Answer: Option B. -> Streptococcus
Question 4. Which of the following inference(s) indicate(s) the ability of an organism to utilize citrate as a sole source of carbon in Simmon's citrate medium?
  1.    Appearance of growth
  2.    Blue colour
  3.    None of these
  4.    Both (a) and (b)
Explanation:-
Answer: Option D. -> Both (a) and (b)
Question 5. The organisms that can be acid-fast stained is
  1.    all of these
  2.    Nocardia
  3.    Lepra bacilli
  4.    Tubercle bacilli
Explanation:-
Answer: Option A. -> all of these
Question 6. The production of sufficient acid by fermentation of glucose leads to decrease in pH such that pH of the medium falls below 4.5. Which of the following test can detect it?
  1.    Methyl red test
  2.    Indole test
  3.    Citrate utilization test
  4.    Voges-Proskauer test
Explanation:-
Answer: Option A. -> Methyl red test
Question 7. At what concentration of sulphuric acid, Nocardia resists decolourization?
  1.    5% sulphuric acid
  2.    8% sulphuric acid
  3.    10% sulphuric acid
  4.    0.5% sulphuric acid
Explanation:-
Answer: Option D. -> 0.5% sulphuric acid
Question 8. Production of acetoin can be detected by which of the following tests?
  1.    Indole test
  2.    Methyl red test
  3.    Citrate test
  4.    Voges-Proskauer test
Explanation:-
Answer: Option D. -> Voges-Proskauer test
Question 9. The primary stain of Gram's method is
  1.    safranin
  2.    phenolpthalene
  3.    crystal violet
  4.    methyl red
Explanation:-
Answer: Option C. -> crystal violet
Question 10. Which of the following is commonly used as Gram's decolouriser?
  1.    A mixture of ethyl alcohol and acetone
  2.    Methyl alcohol
  3.    Ethyl alcohol
  4.    Acetone
Explanation:-
Answer: Option A. -> A mixture of ethyl alcohol and acetone
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