Gas Chromatography(Biochemistry ) Questions and Answers

Question 1. The GC trace obtained after an experiment is called a
  1.    chromatophore
  2.    graph
  3.    chromatograph
  4.    chromatogram
Explanation:-
Answer: Option D. -> chromatogram
Question 2. Which of these effects result from slow injection of a large sample volume?
  1.    Non-linear detector response
  2.    Increased resolution
  3.    Constant resolution
  4.    Decreased resolution
Explanation:-
Answer: Option D. -> Decreased resolution
Question 3. Which of the following is not used for detection in GC?
  1.    Flame ionisation
  2.    NMR
  3.    Infrared spectroscopy
  4.    Electrical conductivity
Explanation:-
Answer: Option B. -> NMR
Question 4. Which of the following detectors give concentration-dependent signals?
  1.    All of these
  2.    Thermal conductivity
  3.    Infra-red detector
  4.    Electron-capture detector
Explanation:-
Answer: Option A. -> All of these
Question 5. What useful information can be found from a Van Deemter plot?
  1.    The selectivity factor
  2.    Optimum mobile phase flow rate
  3.    Optimum column temperature
  4.    Optimum column length
Explanation:-
Answer: Option B. -> Optimum mobile phase flow rate
Question 6. What is the typical internal diameter of fused silica capillary columns?
  1.    0.2-0.3 mm
  2.    1.0-2.0 mm
  3.    0.5-1.0 mm
  4.    0.3-0.5mm
Explanation:-
Answer: Option A. -> 0.2-0.3 mm
Question 7. Derivatisation of a sample is carried out to
  1.    increase the detector response
  2.    increase volatility of the analytes
  3.    All of these
  4.    reduce polarity of the analytes
Explanation:-
Answer: Option C. -> All of these
Question 8. In column switching chromatography
  1.    any of the above
  2.    compounds trapped on one column are eluted to another column
  3.    one column is removed and replaced by another
  4.    the flow to the column is switched on and off repeatedly
Explanation:-
Answer: Option B. -> compounds trapped on one column are eluted to another column
Question 9. Resolution is proportional to the
  1.    number of theoretical plates in a column
  2.    square root of the number of theoretical plates in a column
  3.    cube root of the number of theoretical plates in a column
  4.    square of the number of theoretical plates in a column
Explanation:-
Answer: Option B. -> square root of the number of theoretical plates in a column
Question 10. Which of the statements is correct?
  1.    All of these
  2.    Gas chromatography is used to analyse gases, solutions and solids
  3.    Gas chromatography is used to analyse gases
  4.    Gas chromatography is used to analyse solids
Explanation:-
Answer: Option A. -> All of these
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