Protein Purification(Biochemistry ) Questions and Answers
Question 1. During successful purification scheme, this may be expected that the
number of proteins in the sample decreases
specific activity increases
specific activity decreases
both (a) and (c)
Explanation:-
Answer: Option D. -> both (a) and (c)
Question 2. Affinity chromatography deals with the
None of these
protein - carbohydrate interaction
protein - protein interaction
specific binding of a protein constituents for another molecule
Explanation:-
Answer: Option D. -> specific binding of a protein constituents for another molecule
Question 3. Gel-filtration chromatography separates on the basis of
size and shape using porous beads packed in a column
shape using porous beads packed in a column
None of these
size using porous beads packed in a column
Explanation:-
Answer: Option A. -> size and shape using porous beads packed in a column
Question 4. In ion-exchange chromatography
proteins are separated on the basis of their net charge
either (b) or (c)
proteins are separated on the basis of their size
proteins are separated on the basis of their shape
Explanation:-
Answer: Option A. -> proteins are separated on the basis of their net charge
Question 5. Which of the following may be added to stabilize the protein after yeast cells disruption?
All of these
Protease inhibitor
AMP
NaCl
Explanation:-
Answer: Option A. -> All of these
Question 6. A purified protein sample contains 10 μg of protein and has an enzyme activity of 1 m mole of ATP synthesized/sec (1 unit). What is the specific activity of the final purified sample?
100,000 units/mg
10,000 units/mg.
1,000 units/mg
1,000,000 units/mg
Explanation:-
Answer: Option A. -> 100,000 units/mg
Question 7. The best way to determine the location of protein in the purification scheme is to measure the
mass spectroscopy of the protein
rate of ATP synthesis
changes in the refractive index
UV absorption
Explanation:-
Answer: Option B. -> rate of ATP synthesis
Question 8. Proteins separation can be carried out on the basis of
size or mass
all of these
net charge
solubility in salt solutions
Explanation:-
Answer: Option B. -> all of these
Question 9. During successful purification scheme, this may be expected that the
specific activity increases
specific activity decreases
number of proteins in the sample decreases
both (a) and (c)
Explanation:-
Answer: Option D. -> both (a) and (c)
Question 10. In ion-exchange chromatography
proteins are separated on the basis of their net charge
proteins are separated on the basis of their size
proteins are separated on the basis of their shape
either (b) or (c)
Explanation:-
Answer: Option A. -> proteins are separated on the basis of their net charge