Translation And Regulation(Microbiology ) Questions and Answers

Question 1. Site in the ribosome from which the tRNA donates amino acids to the growing polypeptide chain is
  1.    O site
  2.    A site
  3.    T site
  4.    P site
Explanation:-
Answer: Option D. -> P site
Question 2. Negative regulation of protein synthesis is accomplished by
  1.    the binding of a repressor to the RNA polymerase
  2.    the binding of a repressor to the DNA
  3.    the binding of RNA polymerase to the promoter
  4.    allosteric inhibition
Explanation:-
Answer: Option B. -> the binding of a repressor to the DNA
Question 3. Which binding is inhibited by pactamycin?
  1.    Aminoacyl-tRNA to the A-site of 30S subunit
  2.    Peptidyl t-RNA to the 50S subunit
  3.    Initiator-tRNA to 30S/40S initiation complexes
  4.    Formation of peptide bond in P site
Explanation:-
Answer: Option C. -> Initiator-tRNA to 30S/40S initiation complexes
Question 4. When was the genetic code completed?
  1.    1952
  2.    1968
  3.    1958
  4.    1966
Explanation:-
Answer: Option D. -> 1966
Question 5. Which of the following act as the blueprint or template for the process of protein synthesis that takes place on ribosomes?
  1.    mRNA
  2.    rRNA
  3.    tRNA
  4.    DNA
Explanation:-
Answer: Option A. -> mRNA
Question 6. What is the maximum number of different amino acids in a polypeptide chain coded by the synthetic polyribonucleotides (UCAG)5?
  1.    One
  2.    Two
  3.    Four
  4.    Three
Explanation:-
Answer: Option D. -> Three
Question 7. Which stop codon has been found to encode selenocysteine?
  1.    UGA
  2.    UAA
  3.    AGA
  4.    UAG
Explanation:-
Answer: Option A. -> UGA
Question 8. Which of the following is a nontranslated sequence located between the transcription and the translation start site?
  1.    Leader sequence
  2.    Reading frame
  3.    Ending frame
  4.    Trailer sequence
Explanation:-
Answer: Option A. -> Leader sequence
Question 9. Which phage always carries a small piece of E.coli genome?
  1.    Lambda
  2.    T3
  3.    T4
  4.    Mu
Explanation:-
Answer: Option D. -> Mu
Question 10. The final step of gene expression is protein synthesis, which is also known as
  1.    none of these
  2.    transcription
  3.    replication
  4.    translation
Explanation:-
Answer: Option D. -> translation
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