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Dna Replication(Microbiology ) Questions and Answers
Home
Topic
Microbiology
Dna Replication
Question 1.
Proofreading activity to maintain the fidelity of DNA synthesis
occurs in prokaryotes but not eukaryotes
occurs after the synthesis has been completed
is a function of the 3'-5' exonuclease activity of the DNA polymerases
requires the presence of an enzyme separate from the DNA polymerases
Explanation:-
Answer: Option C. ->
is a function of the 3'-5' exonuclease activity of the DNA polymerases
Question 2.
The enzyme that catalyzes the synthesis of DNA is called
helicase
DNA gyrase
DNA polymerase
DNA ligase
Explanation:-
Answer: Option C. ->
DNA polymerase
Question 3.
Which of the following possesses both 5'-3' and 3'-5' exonuclease activity?
DNA polymerase III
Kornberg enzyme
Taq DNA polymerase
None of these
Explanation:-
Answer: Option B. ->
Kornberg enzyme
Question 4.
Both strands of DNA serve as templates concurrently in
replication
excision repair
mismatch repair
none of these
Explanation:-
Answer: Option A. ->
replication
Question 5.
Which of the following repairs nicked DNA by forming a phosphodiester bond between adjacent nucleotides?
Helicase
DNA gyrase
DNA ligase
Topoisomerases
Explanation:-
Answer: Option C. ->
DNA ligase
Question 6.
Which of the following statements on replication in E.coli is correct?
Always uses T7 DNA polymerase when infected by T7 phage
It occurs in bidirectional manner
It occurs in a unidirectional manner
Occurs only when λ phase has infected E. coli
Explanation:-
Answer: Option B. ->
It occurs in bidirectional manner
Question 7.
DNA gyrase is inhibited by
cephalosporin
both (a) and (b)
nalidixic acid
tetracycline
Explanation:-
Answer: Option C. ->
nalidixic acid
Question 8.
The replication of chromosomes by eukaryotes occurs in a relatively short period of time because
each chromosome contains multiple replicons
the eukaryotic replication machinery is 1000 times faster than the prokaryotes
the eukaryotes have more amount of DNA for replication
eukaryotic DNA is always single stranded
Explanation:-
Answer: Option A. ->
each chromosome contains multiple replicons
Question 9.
During which of the following process a new copy of a DNA molecule is precisely synthesized?
Transcription
Translation
Replication
Trasformation
Explanation:-
Answer: Option C. ->
Replication
Question 10.
In E.coli, which enzyme synthesizes the RNA primer for Okazaki fragments?
all of these
DnaG
DnaA
DnaC
Explanation:-
Answer: Option B. ->
DnaG
1
2
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