Cell Cultivations(Microbiology ) Questions and Answers

Question 1. For most bacteria, the optimum pH for growth lies between
  1.    4.5-5.5
  2.    5.5-6.5
  3.    6.5-7.5
  4.    3.5-4.5
Explanation:-
Answer: Option C. -> 6.5-7.5
Question 2. Thiobacillus thiooxidans has an optimum pH of
  1.    0.5-6.0
  2.    6.5-7.5
  3.    9.0-9.5
  4.    2.0-3.5
Explanation:-
Answer: Option D. -> 2.0-3.5
Question 3. Which of the following is an abiotic elicitors?
  1.    All of these
  2.    Osmotic shock
  3.    Heavy metal ions
  4.    UV irradiation
Explanation:-
Answer: Option A. -> All of these
Question 4. Suspension cultures consist of cells and cell aggregates, growing dispersed in
  1.    solid or liquid medium
  2.    liquid medium
  3.    solid nutrient medium
  4.    none of these
Explanation:-
Answer: Option B. -> liquid medium
Question 5. Which of the following is the necessary step for cultivating the microorganisms?
  1.    Preparing a culture medium for the growth of microorganisms
  2.    All of these
  3.    Inoculating the microorganisms in the prepared medium
  4.    Sterilizing in order to eliminate all living microorganisms in vessel
Explanation:-
Answer: Option B. -> All of these
Question 6. Balanced growth is defined as
  1.    balancing the growth while controlling the temperature
  2.    balancing the growth while changing the nutrient composition
  3.    cultures undergoing balanced growth while maintaining a constant chemical composition
  4.    balancing the growth while controlling the pH
Explanation:-
Answer: Option C. -> cultures undergoing balanced growth while maintaining a constant chemical composition
Question 7. Which of the following is not an indirect method for the measurement of cell mass?
  1.    Nutrient composition
  2.    Viscosity
  3.    Cell dry weight
  4.    Heat evolution
Explanation:-
Answer: Option C. -> Cell dry weight
Question 8. The cell mass can be measured optically by determining the amount of light scattered by a suspension of cells. The measurements are usually at a wavelength of
  1.    300-400nm
  2.    500-600nm
  3.    400-500nm
  4.    600-700nm
Explanation:-
Answer: Option D. -> 600-700nm
Question 9. Callus cultures are amorphous cell aggregates arising from the unorganized growth of explants on an
  1.    liquid medium
  2.    solid nutrient medium
  3.    solid or liquid medium
  4.    aseptic solid nutrient medium
Explanation:-
Answer: Option D. -> aseptic solid nutrient medium
Question 10. Semi-solid media, prepared with agar at concentrations of 0.5% or less are useful for the cultivation of
  1.    E.coli
  2.    Microaerophillic bacteria
  3.    Lactobacilli
  4.    all of these
Explanation:-
Answer: Option B. -> Microaerophillic bacteria
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