Immunological Techniques(Biochemistry ) Questions and Answers

Question 1. Agarose gel electrophoresis and pulsed field gel electrophoresis may be used to resolve respectively
  1.    20 kb and 2000 kb DNA
  2.    2000 kb and 20kb DNA
  3.    10 kb and 1000 kb DNA
  4.    1000 kb and l0kb DNA
Explanation:-
Answer: Option A. -> 20 kb and 2000 kb DNA
Question 2. The IPTG is a gratuitous inducers of (β-galactosidase, and x-gal is a chromogenic substrate. When α-complementation occurs which colour is produced?
  1.    No colour is produced
  2.    White
  3.    Brown
  4.    Blue
Explanation:-
Answer: Option D. -> Blue
Question 3. The competitive immunoassay can be used
  1.    both (a) and (b)
  2.    to detect antibody associated with allergies (IgE)
  3.    commonly to detect trace amounts of drugs.
  4.    to detect very small amounts of antigen
Explanation:-
Answer: Option A. -> both (a) and (b)
Question 4. Which of the following is an immunodiffusion test?
  1.    All of these
  2.    Double-diffusion
  3.    Gel diffusion
  4.    Ouchterloney technique
Explanation:-
Answer: Option A. -> All of these
Question 5. In Counter immunoelectrophoresis
  1.    electrophoresis will drive the antibody and antigen parallel to each other
  2.    electrophoresis will drive the antibody and antigen toward each other
  3.    the antibody will migrate towards cathode
  4.    the antibody will migrate towards anode
Explanation:-
Answer: Option B. -> electrophoresis will drive the antibody and antigen toward each other
Question 6. What is the ELISA test intended to measure?
  1.    Antibodies directed against HLA molecules
  2.    Antibody to HIV only
  3.    Presence of free, circulating virus in the patient
  4.    Antigen to HIV only
Explanation:-
Answer: Option B. -> Antibody to HIV only
Question 7. In Rocket Immunodiffusion the length of the rocket is
  1.    inversely proportional to the amount of antigen placed in each well
  2.    proportional to the amount of antibody placed in each well
  3.    inversely proportional to the amount of antibody placed in each well
  4.    proportional to the amount of antigen placed in each well
Explanation:-
Answer: Option D. -> proportional to the amount of antigen placed in each well
Question 8. Radial Immunodiffusion is similar to
  1.    double-diffusion
  2.    all of these
  3.    gel diffusion
  4.    Ouchterloney technique
Explanation:-
Answer: Option A. -> double-diffusion
Question 9. Immunoaffinity chromatography can be used in biochemical applications to
  1.    break down antibody structure
  2.    None of these
  3.    purify protein antigen
  4.    break down antigen and analyze quantitatively
Explanation:-
Answer: Option C. -> purify protein antigen
Question 10. Different form of a plasmid, on an agarose gel, after electrophoresis may be in which of the following sequence in decreasing molecular weight?
  1.    Linear plasmid, open circular, super coiled plasmid
  2.    Open circular, super coiled, linear plasmid DNA
  3.    Open circular, linear plasmid, super coiled plasmid
  4.    Open circular, super coiled, linear plasmid DNA
Explanation:-
Answer: Option A. -> Linear plasmid, open circular, super coiled plasmid
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