Hplc(Biochemistry ) Questions and Answers

Question 1. HPLC stands for
  1.    High Pressure Liquid Chromatography
  2.    both (a) and (b)
  3.    High Performance Liquid Chromatography
  4.    Highly Placed Liquid Chromatography
Explanation:-
Answer: Option B. -> both (a) and (b)
Question 2. For a typical adsorbent such as silica gel, the most popular pore diameters are
  1.    60 and 100 A°
  2.    10 and 50 A°
  3.    100 and 150 A°
  4.    150 and 200 A°
Explanation:-
Answer: Option A. -> 60 and 100 A°
Question 3. The eluent strength is a measure of
  1.    solvent mixing index
  2.    solvent adsorption energy
  3.    solvent absorption energy
  4.    solvent diffusivity
Explanation:-
Answer: Option B. -> solvent adsorption energy
Question 4. HPLC methods include
  1.    liquid/solid (adsorption) chromatography
  2.    liquid/liquid (partition) chromatography
  3.    ion exchange and size exclusion chromatography
  4.    All of these
Explanation:-
Answer: Option D. -> All of these
Question 5. Column efficiency is measured in terms of number of plates which is
  1.    directly related to the square of the peak width
  2.    inversely related to the cube root of the peak width
  3.    inversely related to the square of the peak width
  4.    directly related to the square of the peak width
Explanation:-
Answer: Option C. -> inversely related to the square of the peak width
Question 6. An isocratic elution in HPLC is one in which the composition of the solvent
  1.    changes in a series of steps
  2.    none of these
  3.    changes continuously
  4.    remains constant
Explanation:-
Answer: Option D. -> remains constant
Question 7. An eluotropic series
  1.    is a measure of the solvent adsorption energy
  2.    None of these
  3.    ranks column packing material by their relative abilities to retain solutes on the column
  4.    ranks solvents by their relative abilities to displace solutes from a given absorbent
Explanation:-
Answer: Option D. -> ranks solvents by their relative abilities to displace solutes from a given absorbent
Question 8. Which of the following statements is true for a refractive index detector in HPLC?
  1.    It does not respond to many solutes
  2.    It can only be used for isocratic elutions
  3.    It is more sensitive than a UV detector
  4.    none of above
Explanation:-
Answer: Option A. -> It does not respond to many solutes
Question 9. A gradient elution in HPLC is one in which the composition of the solvent
  1.    both (a) and (b)
  2.    is changed continuously or in a series of steps
  3.    None of these
  4.    remains constant
Explanation:-
Answer: Option B. -> is changed continuously or in a series of steps
Question 10. In reversed phase HPLC, there is a
  1.    non polar solvent/polar column
  2.    polar solvent/non-polar column
  3.    any of the above
  4.    non polar solvent/non-polar column
Explanation:-
Answer: Option B. -> polar solvent/non-polar column
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