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Gel Electrophoresis(Biochemistry ) Questions and Answers
Home
Topic
Biochemistry
Gel Electrophoresis
Question 1.
Electrophoresis of histones and myoglobin under non-denaturing conditions (pH = 7.0) results in
both proteins migrate to the cathode
histones migrate to the cathode and myoglobin migrates to the anode
both proteins migrate to the anode
histones migrate to the anode and myoglobin migrates to the cathode
Explanation:-
Answer: Option B. ->
histones migrate to the cathode and myoglobin migrates to the anode
Question 2.
Proteins can be visualized directly in gels by
using electron microscope only
measuring their molecular weight
staining them with the dye
none of these
Explanation:-
Answer: Option C. ->
staining them with the dye
Question 3.
In an SDS-PAGE
proteins have the same charge-to-mass ratio
smaller proteins migrate more rapidly through the gel
All of these
proteins are denatured by the SDS
Explanation:-
Answer: Option C. ->
All of these
Question 4.
In isoelectric focusing, proteins are separated on the basis of their
relative content of positively and negatively charged residue
size
relative content of negatively charged residue only
relative content of positively charged residue only
Explanation:-
Answer: Option A. ->
relative content of positively and negatively charged residue
Question 5.
In SDS-PAGE, the protein sample is first
treated with a oxidizing agent and then with anionic detergent followed by fractionation by electrophoresis
fractionated by electrophoresis then treated with an oxidizing agent followed by anionic detergent.
None of these
treated with a reducing agent and then with anionic detergent followed by fractionation by electrophoresis
Explanation:-
Answer: Option D. ->
treated with a reducing agent and then with anionic detergent followed by fractionation by electrophoresis
Question 6.
In a gel filtration column
large proteins enter the beads more readily
smaller proteins enter the beads more readily
large proteins elute first
both (a) and (b)
Explanation:-
Answer: Option D. ->
both (a) and (b)
Question 7.
Proteins are separated in an SDS-PAGE experiment on the basis of their
positively charged side chains
negatively charged side chains
molecular weight
different isoelectric points
Explanation:-
Answer: Option C. ->
molecular weight
Question 8.
In a native PAGE, proteins are separated on the basis of
net positive charge
net negative charge
net positive charges size
net charge and size
Explanation:-
Answer: Option D. ->
net charge and size
Question 9.
The subunit molecular weight as well as the number of subunits in the quaternary structure can be determined by
isoelectric focusing
SDS-PAGE electrophoresis
combining information from (a)and (b)
gel filtration chromatography
Explanation:-
Answer: Option C. ->
combining information from (a)and (b)
Question 10.
In an SDS-PAGE
proteins are denatured by the SDS
proteins have the same charge-to-mass ratio
smaller proteins migrate more rapidly through the gel
all of the above
Explanation:-
Answer: Option D. ->
all of the above
1
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