Plant Cell Culture(Biotechnology ) Questions and Answers

Question 1. Which method of plant propagation involves the use of girdling?
  1.    Grafting
  2.    Layering
  3.    Cuttings
  4.    Micropropagation
Explanation:-
Answer: Option B. -> Layering
Question 2. A(n) __________ is an excised piece of leaf or stem tissue used in micropropagation.
  1.    explant
  2.    medium
  3.    scion
  4.    microshoot
Explanation:-
Answer: Option A. -> explant
Question 3. Protoplasts can be produced from suspension cultures, callus tissues or intact tissues by enzymatic treatment with
  1.    pectolytic enzymes
  2.    proteolytic enzymes
  3.    both cellulotyic and pectolytic enzymes
  4.    cellulotyic enzymes
Explanation:-
Answer: Option C. -> both cellulotyic and pectolytic enzymes
Question 4. Which of the following is considered as the disadvantage of conventional plant tissue culture for clonal propagation?
  1.    Storage and transportation of propogates
  2.    Both (b) and (c)
  3.    Less multiplication of disease free plants
  4.    Multiplication of sexually derived sterile hybrids
Explanation:-
Answer: Option A. -> Storage and transportation of propogates
Question 5. What is meant by 'Organ culture' ?
  1.    Maintenance alive of a whole organ, after removal from the organism by partial immersion in a nutrient fluid
  2.    The aspects of culture in community which are mainly dedicated by the need of a specified organ of the human body
  3.    Cultivation of organs in a laboratory through the synthesis of tissues
  4.    Introduction of a new organ in an animal body with a view to create genetic mutation in the progenies of that animal
Explanation:-
Answer: Option A. -> Maintenance alive of a whole organ, after removal from the organism by partial immersion in a nutrient fluid
Question 6. Organogenesis is
  1.    genesis of organs
  2.    formation of root and shoots on callus tissue
  3.    both (a) and (b)
  4.    formation of callus tissue
Explanation:-
Answer: Option B. -> formation of root and shoots on callus tissue
Question 7. In a callus culture
  1.    auxins and cytokinins are not required
  2.    increasing level of cytokinin to a callus induces shoot formation and increasing level of auxin promote root formation
  3.    increasing level of auxin to a callus induces shoot formation and increasing level of cytokinin promote root formation
  4.    only auxin is required for root and shoot formation
Explanation:-
Answer: Option B. -> increasing level of cytokinin to a callus induces shoot formation and increasing level of auxin promote root formation
Question 8. Which breeding method uses a chemical to strip the cell wall of plant cells of two sexually incompatible species?
  1.    Mass selection
  2.    Transpiration
  3.    Transformation
  4.    Protoplast fusion
Explanation:-
Answer: Option D. -> Protoplast fusion
Question 9. Protoplasts are the cells devoid of
  1.    none of these
  2.    cell wall
  3.    both cell wall and cell membrane
  4.    cell membrane
Explanation:-
Answer: Option B. -> cell wall
Question 10. Which of the following is used in the culture of regenerating protoplasts, single cells or very dilute cell suspensions?
  1.    Nurse medium
  2.    Both (a) and (b)
  3.    Nurse or feeder culture
  4.    None of these
Explanation:-
Answer: Option B. -> Both (a) and (b)
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