Media Required For Plant Cell Culture(Biotechnology ) Questions and Answers

Question 1. Which of the following growth regulator is added for short initiation during plant regeneration from callus?
  1.    Brassinosteroids
  2.    Cytokinins
  3.    Gibberellins
  4.    Auxins
Explanation:-
Answer: Option B. -> Cytokinins
Question 2. Which of the following is an ethylene biosynthesis inhibitor?
  1.    Activated charcoal
  2.    Citric acid
  3.    Silver thiosulphate
  4.    Succinic acid
Explanation:-
Answer: Option C. -> Silver thiosulphate
Question 3. Which is the most common carbon source used in the plant cell culture media?
  1.    Glucose
  2.    Fructose
  3.    Maltose
  4.    Sucrose
Explanation:-
Answer: Option D. -> Sucrose
Question 4. Nitrogen in the plant cell culture media is provided by either ammonia or nitrate salt. In the media
  1.    utilization of ammonium cause culture pH to drop while utilization of nitrate cause culture pH to rise
  2.    utilization of both ammonium and nitrate result in rise in pH
  3.    utilization of both ammonium and nitrate result in drop in pH
  4.    utilization of nitrate cause culture pH to drop while utilization of ammonium cause culture pH to rise
Explanation:-
Answer: Option A. -> utilization of ammonium cause culture pH to drop while utilization of nitrate cause culture pH to rise
Question 5. Which of the following growth regulator promote cell division?
  1.    Cytokinins
  2.    Auxins
  3.    Gibberellins
  4.    Brassinosteroids
Explanation:-
Answer: Option A. -> Cytokinins
Question 6. Concentration of sucrose generally used in plant cell culture media is
  1.    10-15 g/l
  2.    40-50 g/l
  3.    20-30 g/l
  4.    60-70 g/l
Explanation:-
Answer: Option C. -> 20-30 g/l
Question 7. Which of the following growth regulator cause plant cells to grow?
  1.    Brassinosteroids
  2.    Gibberellins
  3.    Cytokinins
  4.    Auxins
Explanation:-
Answer: Option D. -> Auxins
Question 8. Silver thiosulphate is added to culture medium as it helps to
  1.    prevent the gaseous plant hormone, ethylene dioxide from accumulating to detrimental condition.
  2.    remove toxic phenolics from plant cells
  3.    maintain the pH
  4.    All of these
Explanation:-
Answer: Option A. -> prevent the gaseous plant hormone, ethylene dioxide from accumulating to detrimental condition.
Question 9. In plant cell culture media, auxins and cytokinins are used in the range of
  1.    100-125μM
  2.    l-50μM
  3.    50-100μM
  4.    more than 125μM
Explanation:-
Answer: Option B. -> l-50μM
Question 10. Which of the following growth regulator is used to stimulate embryo or shoot development?
  1.    Brassinosteroids
  2.    Gibberellins
  3.    Cytokinins
  4.    Auxins
Explanation:-
Answer: Option B. -> Gibberellins
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