Polymerase Chain Reaction In Biochemistry(Biochemistry ) Questions and Answers

Question 1. The viruses that can achieve neoplastic transformation are called
  1.    retroviruses HIV
  2.    none of these
  3.    RNA tumor viruses
  4.    DNA tumor viruses
Explanation:-
Answer: Option D. -> DNA tumor viruses
Question 2. Genomic libraries are made from
  1.    genomic RNA of an organism
  2.    genomic DNA of an organism
  3.    genomic mRNA of an organism
  4.    genomic cDNA of an organism
Explanation:-
Answer: Option B. -> genomic DNA of an organism
Question 3. From a single molecule of DNA, PCR can make
  1.    millions of copies
  2.    thousands of copies
  3.    one additional copy
  4.    hundreds of copies
Explanation:-
Answer: Option A. -> millions of copies
Question 4. For gene transfer to be effective, transforming DNA must be
  1.    None of these
  2.    incorporated into a viral genome
  3.    incorporated into the bacterial chromosome
  4.    free in the bacterial cytoplasm
Explanation:-
Answer: Option C. -> incorporated into the bacterial chromosome
Question 5. Double stranded DNA denaturation with specified limit of temperature is
  1.    either (a) or (b)
  2.    none of these
  3.    reversible reaction
  4.    irreversible reaction
Explanation:-
Answer: Option C. -> reversible reaction
Question 6. Bacteriophages are
  1.    bacterial viruses
  2.    cells in the blood that eat bacteria
  3.    a class of bacteria
  4.    None of these
Explanation:-
Answer: Option A. -> bacterial viruses
Question 7. Specialized transduction occurs when
  1.    the bacteriophage incorporates randomly in the bacterial chromosome
  2.    None of these
  3.    the bacteriophage never incorporates into the bacterial chromosome
  4.    the bacteriophage always incorporates at the same position in the bacterial chromosome
Explanation:-
Answer: Option D. -> the bacteriophage always incorporates at the same position in the bacterial chromosome
Question 8. PCR can be used to amplify a specific fragment of DNA from which of the following?
  1.    A fragment of skin
  2.    A hair follicle
  3.    A drop of blood
  4.    All of these
Explanation:-
Answer: Option D. -> All of these
Question 9. PCR is used
  1.    to diagnose genetic diseases
  2.    to solve crimes
  3.    to study gene function
  4.    all of these
Explanation:-
Answer: Option D. -> all of these
Question 10. To clone into a plasmid vector, both the plasmid and the foreign DNA are cut
  1.    with the combination of enzymes and then seperated
  2.    with the combination of enzymes and mixed together
  3.    with the same restriction enzyme and mixed together
  4.    with different restriction enzyme and mixed together
Explanation:-
Answer: Option C. -> with the same restriction enzyme and mixed together
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