Recombinant Dna(Biotechnology ) Questions and Answers

Question 1. The deliberate modifications of an organism's genetic information by directly changing its nucleic acid content is a subject matter of
  1.    genetic engineering
  2.    population genetics
  3.    microbiology
  4.    protein engineering
Explanation:-
Answer: Option A. -> genetic engineering
Answer: (a).genetic engineering
Question 2. Which of the following is obtained using processed mRNA molecules as a template?
  1.    rDNA
  2.    mDNA
  3.    cDNA
  4.    tDNA
Explanation:-
Answer: Option C. -> cDNA
Answer: (c).cDNA
Question 3. What is the normal role of restriction endonucleases in bacterial cells?
  1.    To degrade the bacterial chromosome into small pieces during replication
  2.    To degrade invading phage DNA
  3.    To produce RNA primers for replication
  4.    All of the above
Explanation:-
Answer: Option B. -> To degrade invading phage DNA
Answer: (b).To degrade invading phage DNA
Question 4. Vectors are
  1.    molecules that degrade nucleic acids
  2.    molecules that help in replication
  3.    molecules that are able to covalently bond to and carry foreign DNA into cells
  4.    molecules that protect host cells from invasion by foreign DNA
Explanation:-
Answer: Option C. -> molecules that are able to covalently bond to and carry foreign DNA into cells
Answer: (c).molecules that are able to covalently bond to and carry foreign DNA into cells
Question 5. Electroporation is
  1.    the process of separating charged molecules through a gel maintained in an electric field
  2.    the process of combining foreign DNA to an electrically charged vector molecule
  3.    the application of high voltage pulses
  4.    the process of multiplication of the cells
Explanation:-
Answer: Option C. -> the application of high voltage pulses
Answer: (c).the application of high voltage pulses
Question 6. The piece of equipment, that introduces DNA into cells via DNA-coated microprojectiles is known as
  1.    laser
  2.    DNA probe
  3.    gene gun
  4.    inoculating needle
Explanation:-
Answer: Option C. -> gene gun
Answer: (c).gene gun
Question 7. An animal, that has gained new genetic information from the acquisition of foreign DNA, is considered as
  1.    a chimera
  2.    a transgenic animal
  3.    a vector
  4.    an enzyme that links DNA molecules
Explanation:-
Answer: Option B. -> a transgenic animal
Answer: (b).a transgenic animal
Question 8. The advantage of using DNA polymerases from thermophilic organisms in PCR is that
  1.    the DNA polymerases of these bacteria are much faster than those from other organisms
  2.    the DNA polymerases of these bacteria can withstand the high temperatures needed to denature the DNA strands
  3.    the DNA polymerases of these bacteria never make mistakes while replicating DNA
  4.    all of the above
Explanation:-
Answer: Option B. -> the DNA polymerases of these bacteria can withstand the high temperatures needed to denature the DNA strands
Answer: (b).the DNA polymerases of these bacteria can withstand the high temperatures needed to denature the DNA strands
Question 9. Which of the following is not commonly used as vector?
  1.    Artificial chromosome
  2.    Cosmid
  3.    Fungi
  4.    Plasmid
Explanation:-
Answer: Option C. -> Fungi
Answer: (c).Fungi
Question 10. A short molecule containing 2-20 nucleotide is
  1.    plasmid
  2.    vector
  3.    oligonucleotide
  4.    mononucleotide
Explanation:-
Answer: Option C. -> oligonucleotide
Answer: (c).oligonucleotide
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