Immobilized Enzymes Kinetics(Enzyme Technology ) Questions and Answers

Question 1. The plot in the following diagram are referred to as _______________The Plot In The Following Diagram Are Referred To As _______...
  1.    concentration profile
  2.    pH profile
  3.    arrhenius plot
  4.    hanes plot
Explanation:-
Answer: Option C. -> arrhenius plot
Answer: (c).arrhenius plot
Question 2. What does ‘X’ represent in the following graph?What Does ‘X’ Represent In The Following Graph?
  1.    Turnover number
  2.    Damkohler number
  3.    Vₘₐₓ
  4.    Effectiveness factor
Explanation:-
Answer: Option D. -> Effectiveness factor
Answer: (d).Effectiveness factor
Question 3. What does the diagram represent?What Does The Diagram Represent?
  1.    The effect of facilitated transport of hydrogen ions
  2.    Effect of substrate inhibition
  3.    The effect of diffusional control on pH activity profile of an immobilized enzyme
  4.    The effect of diffusional control on the local pH
Explanation:-
Answer: Option A. -> The effect of facilitated transport of hydrogen ions
Answer: (a).The effect of facilitated transport of hydrogen ions
Question 4. What does the graph predict?What Does The Graph Predict?
  1.    Effect of pH on activity of enzyme
  2.    Effect of substrate inhibition
  3.    Effect of temperature on activity on enzyme
  4.    Arrhenius plot
Explanation:-
Answer: Option B. -> Effect of substrate inhibition
Answer: (b).Effect of substrate inhibition
Question 5. What does the following diagram represent?What Does The Following Diagram Represent?
  1.    Activity profile of immobilized enzymes against pH
  2.    Lineweaver Burk plot
  3.    Effect of multipoint interaction for stabilization of enzymes
  4.    Effect of temperature on activity of enzymes
Explanation:-
Answer: Option A. -> Activity profile of immobilized enzymes against pH
Answer: (a).Activity profile of immobilized enzymes against pH
Question 6. Pick the odd one out.
  1.    Inertness of the carrier matrix
  2.    Matrix should be cheap
  3.    Repeated use of enzymes
  4.    Matrix should not allow microbial contamination
Explanation:-
Answer: Option C. -> Repeated use of enzymes
Answer: (c).Repeated use of enzymes
Question 7. The surface of the matrix on which an enzyme is immobilized is called __________
  1.    enzyme immobilization
  2.    adsorption
  3.    biosensor
  4.    carrier matrix
Explanation:-
Answer: Option D. -> carrier matrix
Answer: (d).carrier matrix
Question 8. Which of the following is a disadvantage of immobilized enzymes over free enzymes?
  1.    Repeated use of enzymes
  2.    Minimal downstream processing
  3.    Enzyme may become inactive
  4.    Thermal stability of the enzyme increases
Explanation:-
Answer: Option C. -> Enzyme may become inactive
Answer: (c).Enzyme may become inactive
Question 9. The immobilization method in which the enzyme is linked to the carrier matrix by covalent bond between them is referred to as __________
  1.    adsorption
  2.    covalent binding
  3.    entrapment
  4.    membrane confinement
Explanation:-
Answer: Option B. -> covalent binding
Answer: (b).covalent binding
Question 10. Which of the following is not an example of adsorbent?
  1.    Clay
  2.    Sepharose
  3.    Porous carbon
  4.    Ion-exchange matrices
Explanation:-
Answer: Option B. -> Sepharose
Answer: (b).Sepharose
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