Recombinant Dna Technology(Biochemistry ) Questions and Answers

Question 1. The RNA polymerases that transcribe bacterial DNA are
  1.    multisubunit enzymes
  2.    only active inside the cell
  3.    interchangable with DNA polymerases
  4.    highly glycosylated in their active forms
Explanation:-
Answer: Option A. -> multisubunit enzymes
Answer: (a).multisubunit enzymes
Question 2. The first protein complex to bind to the TATA box is
  1.    transcription factor IIA
  2.    transcription factor IIB
  3.    transcription factor IID
  4.    all of these
Explanation:-
Answer: Option C. -> transcription factor IID
Answer: (c).transcription factor IID
Question 3. Sex lethal (sxl) in Drosophila is transcribed when
  1.    the X autosome balance is less than 0.5
  2.    the X autosome balance is 1 or greater
  3.    the X autosome balance equals 0.5
  4.    none of the above
Explanation:-
Answer: Option B. -> the X autosome balance is 1 or greater
Answer: (b).the X autosome balance is 1 or greater
Question 4. Immediately after transcription
  1.    a methylated guanine cap is added to the 51 end of the transcript
  2.    a methylated guanine cap is added to the 31 end of the transcript
  3.    both (a) and (b)
  4.    a poly adenylation signal is added
Explanation:-
Answer: Option A. -> a methylated guanine cap is added to the 51 end of the transcript
Answer: (a).a methylated guanine cap is added to the 51 end of the transcript
Question 5. A mutation in the tip binding site of the repressor would result in
  1.    constitutive trp operon expression
  2.    inducible trp operon expression
  3.    no operon expression
  4.    none of the above
Explanation:-
Answer: Option A. -> constitutive trp operon expression
Answer: (a).constitutive trp operon expression
Question 6. Enhancers differ from promoters in that
  1.    they initiate transcription
  2.    they are adjacent to the start codon
  3.    their orientation can be inverted without effect
  4.    they are restricted to a specific gene
Explanation:-
Answer: Option C. -> their orientation can be inverted without effect
Answer: (c).their orientation can be inverted without effect
Question 7. A frameshift mutation occurs in the sigma subunit gene transcribed as part of SPOl early genes. What will be the immediate result?
  1.    Early genes will not be translated
  2.    Middle genes will not be transcribed
  3.    The bacterial host will lyse immediately
  4.    Infection will proceed as usual
Explanation:-
Answer: Option C. -> The bacterial host will lyse immediately
Answer: (c).The bacterial host will lyse immediately
Question 8. Methylated gene are
  1.    active
  2.    silent
  3.    dynamic
  4.    either (a) or (b)
Explanation:-
Answer: Option B. -> silent
Answer: (b).silent
Question 9. Tubulin is regulated by
  1.    binding of tubulin to tubulin mRNA
  2.    splicing of the tubulin transcript
  3.    binding of tubulin to the tubulin translational product
  4.    binding of tubulin to DNA
Explanation:-
Answer: Option C. -> binding of tubulin to the tubulin translational product
Answer: (c).binding of tubulin to the tubulin translational product
Question 10. In the presence of high levels of tryptophan
  1.    attenuator allows transcription of trp structural genes
  2.    attenuator propogates transcription
  3.    attenuator terminates transcription
  4.    none of the above
Explanation:-
Answer: Option C. -> attenuator terminates transcription
Answer: (c).attenuator terminates transcription
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