Immobilized Enzyme(Engineering > Biochemical Engineering ) Questions and Answers

Question 1. The support material for immobilization of cells of Bacillus subtilis is
  1.    ion exchange resins
  2.    gelatin
  3.    Anthracite
  4.    agarose and carbodiimide
Explanation:-
Answer: Option D. -> agarose and carbodiimide
Answer: (d).agarose and carbodiimide
Question 2. Which of the following is not a physical method of immobilization?
  1.    Adsorption
  2.    Entrapment
  3.    Micro encapsulation
  4.    None of these
Explanation:-
Answer: Option D. -> None of these
Answer: (d).None of these
Question 3. The intraparticle mass transfer resistance can affect the rate of enzyme reaction, if enzymes are immobilized by
  1.    copolymerization or microencapsulation
  2.    crosslinking using multifunctional reagents
  3.    adsorption
  4.    all of the above
Explanation:-
Answer: Option A. -> copolymerization or microencapsulation
Answer: (a).copolymerization or microencapsulation
Question 4. The covalent attachment of enzyme molecules is via
  1.    nonessential amino acids residues to water insoluble, functional supports
  2.    essential amino acids residues to water insoluble, functional supports
  3.    nonessential amino acids residues to water soluble, functional supports
  4.    essential amino acids residues to water soluble, functional supports
Explanation:-
Answer: Option A. -> nonessential amino acids residues to water insoluble, functional supports
Answer: (a).nonessential amino acids residues to water insoluble, functional supports
Question 5. To measure the extent to which the reaction rate is lowered because of resistance to mass transfer, the effectiveness factor of an immobilized enzyme, η can be defined as
  1.    reaction rate/rate if not slowed by diffusion
  2.    rate if not slowed by diffusion/ reaction rate
  3.    actual reaction rate/ rate if not slowed by diffusion
  4.    rate if not slowed by diffusion/ actual reaction rate
Explanation:-
Answer: Option C. -> actual reaction rate/ rate if not slowed by diffusion
Answer: (c).actual reaction rate/ rate if not slowed by diffusion
Question 6. Which of the following is considered as a disadvantage to the adsorption method of immobilization?
  1.    It is possible to separate and purify the enzymes while being immobilized
  2.    The enzymes are not usually deactivated by adsorption
  3.    The adsorption is a reversible process
  4.    State of immobilization is very sensitive to solution pH, ionic strength and temperature
Explanation:-
Answer: Option D. -> State of immobilization is very sensitive to solution pH, ionic strength and temperature
Answer: (d).State of immobilization is very sensitive to solution pH, ionic strength and temperature
Question 7. During the enzymatic reaction of an immobilized enzyme, the rate of substrate transfer is
  1.    equal to that of substrate consumption
  2.    more than that of substrate consumption
  3.    lesser than that of substrate consumption
  4.    is nothing to do with the substrate consumption
Explanation:-
Answer: Option A. -> equal to that of substrate consumption
Answer: (a).equal to that of substrate consumption
Question 8. The immobilized technique involving chemical method is
  1.    covalent bond formation dependent
  2.    non-covalent bond formation dependent
  3.    both (a) and (b)
  4.    ionic bond formation dependent
Explanation:-
Answer: Option A. -> covalent bond formation dependent
Answer: (a).covalent bond formation dependent
Question 9. Damkohler number(NDa) is
  1.    the ratio of the maximum reaction rate to the maximum mass transfer rate
  2.    the ratio of the minimum reaction rate to the maximum mass transfer rate
  3.    the ratio of the maximum reaction rate to the minimum mass transfer rate
  4.    the ratio of the minimum reaction rate to the minimum mass transfer rate
Explanation:-
Answer: Option A. -> the ratio of the maximum reaction rate to the maximum mass transfer rate
Answer: (a).the ratio of the maximum reaction rate to the maximum mass transfer rate
Question 10. For a steady state condition, the change of substrate concentration (dCs/dt) is
  1.    zero
  2.    1
  3.    >1
  4.    <1
Explanation:-
Answer: Option A. -> zero
Answer: (a).zero
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