Modifications And Mutagenesis(Engineering > Genetic Engineering ) Questions and Answers
Question 1. Double stranded RNA is cleaved by a nuclease called as Dicer and small fragments are generated known as ___________
short interfering RNAs
long interfering RNAs
short interspersed RNAs
long interspersed RNAs
Explanation:-
Answer: Option A. -> short interfering RNAs Answer: (a).short interfering RNAs
Question 2. Down regulation of expression of endogenous genes by transformation with constructs that would generate sense RNA, rather than anti-sense RNA is known as ___________
suppression
co-suppression
multisuppression
anti-suppression
Explanation:-
Answer: Option B. -> co-suppression Answer: (b).co-suppression
Question 3. In some organisms, presence of double stranded RNAs leads to breakdown of corresponding single stranded mRNA.
True
False
May be True or False
Can't say
Explanation:-
Answer: Option A. -> True Answer: (a).True
Question 4. DNA _________ is also a method for gene silencing through short RNAs.
acetylation
phosphorylation
methylation
acylation
Explanation:-
Answer: Option C. -> methylation Answer: (c).methylation
Question 5. The process of RNA inactivation by siRNAs is termed as ___________
RNA silencing
RNA interference
Short RNA inactivation
RNA disfunction
Explanation:-
Answer: Option B. -> RNA interference Answer: (b).RNA interference
Question 6. Choose the correct statement for self cleavage reaction.
It is nucleophilic attack reaction by 2’ hydroxyl of RNA
It is nucleophilic attack reaction by 3’ hydroxyl of RNA
It is nucleophilic attack reaction by 2’ hydroxyl of DNA
It is nucleophilic attack reaction by 3’ hydroxyl of DNA
Explanation:-
Answer: Option A. -> It is nucleophilic attack reaction by 2’ hydroxyl of RNA Answer: (a).It is nucleophilic attack reaction by 2’ hydroxyl of RNA
Question 7. A substance that induces unregulated growth processes in cells or tissues leading to cancer are called as
mutagen
carcinogen
Pathogens
Microbes
Explanation:-
Answer: Option B. -> carcinogen Answer: (b).carcinogen
Question 8. Self cleavage reaction can take place in?
DNA
RNA
Both DNA and RNA
Can take place in both but is preferred in DNA
Explanation:-
Answer: Option B. -> RNA Answer: (b).RNA
Question 9. Mutagens can be
Interferes the functions of nucleotides
Physical agent
Chemical agent
all of above
Explanation:-
Answer: Option D. -> all of above Answer: (d).all of above
Question 10. Carcinogens are always
mutagens
mutants
point mutants
silent mutant
Explanation:-
Answer: Option A. -> mutagens Answer: (a).mutagens