Enzyme Assay And Enzyme Catalysis(Enzyme Technology ) Questions and Answers

Question 1. Continuous assay: Glucose-6-phosphate dehydrogenase:: Luminescence method: ____________
  1.    Hydrolases
  2.    Bacterial luciferase
  3.    Ornithine decarboxylase
  4.    Glutamate decarboxylase
Explanation:-
Answer: Option B. -> Bacterial luciferase
Answer: (b).Bacterial luciferase

Question 2. In which of the following methods, the intensity of emitted light is used to study enzyme reaction?
  1.    Discontinuous assay
  2.    Luminescence method
  3.    Biosensors
  4.    Spectrophotometer
Explanation:-
Answer: Option C. -> Biosensors
Answer: (c).Biosensors

Question 3. Which of the following is not true for luminescence method?
  1.    The intensity of emitted light is used to study enzyme reaction
  2.    The reaction can be used to assay ATP and appropriate enzymes via coupled reaction
  3.    Bacterial luciferase can be assayed and measured by this method
  4.    It is based on stoke shift
Explanation:-
Answer: Option D. -> It is based on stoke shift
Answer: (d).It is based on stoke shift

Question 4. Which of the following is true for radioisotope method?
  1.    The intensity of emitted light is used to study enzyme reaction
  2.    The reaction can be used to assay ATP and appropriate enzymes via coupled reaction
  3.    It is restricted to separation of radiolabeled forms of substrate and product
  4.    Bacterial luciferase can be assayed and measured by this method
Explanation:-
Answer: Option C. -> It is restricted to separation of radiolabeled forms of substrate and product
Answer: (c).It is restricted to separation of radiolabeled forms of substrate and product

Question 5. Which of the following is not true for radioisotope method?
  1.    This method is used in studying mechanism of enzyme action and in ligand binding sites
  2.    Change is absorbance is used as the basis for assaying enzyme
  3.    If one product is gas, the method is easier
  4.    Glutamate decarboxylase is assayed and measured using this method
Explanation:-
Answer: Option B. -> Change is absorbance is used as the basis for assaying enzyme
Answer: (b).Change is absorbance is used as the basis for assaying enzyme

Question 6. The lab methods for measuring enzymatic activity for the study of enzyme kinetic and enzyme inhibition is referred to as _________
  1.    enzyme reactors
  2.    entrapment
  3.    biosensors
  4.    enzyme assay
Explanation:-
Answer: Option D. -> enzyme assay
Answer: (d).enzyme assay

Question 7. SI unit of enzyme activity is _________
  1.    mol
  2.    m/s
  3.    katal
  4.    Newton
Explanation:-
Answer: Option C. -> katal
Answer: (c).katal

Question 8. ____________ is a measure of the quantity of active enzyme present which depends on condition.
  1.    Kₘ
  2.    Vₘₐₓ
  3.    Enzyme activity
  4.    Turn over number
Explanation:-
Answer: Option C. -> Enzyme activity
Answer: (c).Enzyme activity

Question 9. 1 U = _______ nanokatals.
  1.    16.67
  2.    3.14
  3.    9.8
  4.    273
Explanation:-
Answer: Option A. -> 16.67
Answer: (a).16.67

Question 10. Enzyme can tolerate extreme high salt concentration.
  1.    True
  2.    False
  3.    May be True or False
  4.    Can't say
Explanation:-
Answer: Option B. -> False
Answer: (b).False