Enzyme Technology Future Prospects(Enzyme Technology ) Questions and answers for exam Preparation

Question 1. Which of the following synzyme has 27% of the activity of a-chymotrypsin against 4-nitrophenyl esters?

Primary amines alkylated with 1-iodododecane and 4(5)-chloromethylimidazole
Myoglobin achieved by attaching (Ru(NH₃)₅)³⁺ to three surface histidine residues
C-6 hydroxyl group of β-cyclodextrins was covalently derivatized by an activated pyridoxal coenzyme
Polyglutamic acid acts as an esterase in much the same fashion as the acid proteases for the hydrolysis of 4-nitrophenyl acetate

Explanation:-

Answer: Option A. -> Primary amines alkylated with 1-iodododecane and 4(5)-chloromethylimidazole
Answer: (a).Primary amines alkylated with 1-iodododecane and 4(5)-chloromethylimidazole

Question 2. Coenzyme may be immobilised together with the enzyme and regenerated in situ.

True
False
May be True or False
Can't say

Explanation:-

Answer: Option A. -> True
Answer: (a).True

Question 3. Which of the following is not a strategy to produce synzymes?

Monoclonal antibodies covalently attached to the phosphonate esters which can act as esterases
Molecular imprinting of polymers
Primary amines alkylated with 1-iodododecane and 4(5)-chloromethylimidazole
Synthesis of cyclodextrins from starch by the cyclomaltodextrin glucanotransferase from Bacillus macerans

Explanation:-

Answer: Option D. -> Synthesis of cyclodextrins from starch by the cyclomaltodextrin glucanotransferase from Bacillus macerans
Answer: (d).Synthesis of cyclodextrins from starch by the cyclomaltodextrin glucanotransferase from Bacillus macerans

Question 4. Which of the following is not a coenzyme?

NADH
ATP
Synzyme
NADPH

Explanation:-

Answer: Option C. -> Synzyme
Answer: (c).Synzyme

Question 5. Which of the following is not true for coenzymes?

Coenzymes may be immobilized/regenerated with the use of whole-cell systems
Membrane reactors may be used to immobilize the coenzyme
They are referred to as artificial enzymes
Coenzymes may be derivatized for adequate immobilization and regeneration

Explanation:-

Answer: Option C. -> They are referred to as artificial enzymes
Answer: (c).They are referred to as artificial enzymes

Question 6. Which of the following coenzyme is not derivatized by alkylation of the exocyclic N6-amino nitrogen of the adenine present in it?

Papain
Coenzyme A
NAD⁺
NADP⁺

Explanation:-

Answer: Option A. -> Papain
Answer: (a).Papain

Question 7. Which of the following is an example of regeneration of the derivatized coenzymes by enzymic means?

Formate dehydrogenase
Exocyclic N6-amino nitrogen of the adenine moiety
Dextrans
Polyethylene glycol

Explanation:-

Answer: Option A. -> Formate dehydrogenase
Answer: (a).Formate dehydrogenase

Question 8. What is represented in the following diagram?

Protein engineering cycle
Site directed mutagenesis
Polymerase chain reaction
Transcription

Explanation:-

Answer: Option A. -> Protein engineering cycle
Answer: (a).Protein engineering cycle

Question 9. Which of the following are high molecular weight water-soluble derivatives which are used for derivatization of co-enzymes?

Dextrans
Exocyclic N6-amino nitrogen
Polyethyleneimine
Polyethylene glycols

Explanation:-

Answer: Option B. -> Exocyclic N6-amino nitrogen
Answer: (b).Exocyclic N6-amino nitrogen

Question 10. Which of the following system is not used to derivatize coenzymes?

Alkylation of the exocyclic N6-amino nitrogen of the adenine moiety present in the coenzymes
High molecular weight water-soluble derivatives
Derivative of myoglobin acts as an effective natural ascorbate oxidase
Enzymic regeneration

Explanation:-

Answer: Option C. -> Derivative of myoglobin acts as an effective natural ascorbate oxidase
Answer: (c).Derivative of myoglobin acts as an effective natural ascorbate oxidase