Techniques In Cell Biology(Cell Biology ) Questions and Answers

Question 1. In mass-spectrometry, proteins are separated base on their _____________________
  1.    i-value
  2.    c-value
  3.    m/z ratio
  4.    e/m ratio
Explanation:-
Answer: Option C. -> m/z ratio
Answer: (c).m/z ratio
Question 2. MALDI is a technique of ______________________
  1.    ionization
  2.    fractionation
  3.    proteolysis
  4.    cell counting
Explanation:-
Answer: Option A. -> ionization
Answer: (a).ionization
Question 3. Electrospray ionization is suited for proteins that are __________________
  1.    in matrix
  2.    in glycerol
  3.    in solution
  4.    of large size
Explanation:-
Answer: Option C. -> in solution
Answer: (c).in solution
Question 4. Which is the main ingredient in the sample preparation of mass spectrometry?
  1.    papain
  2.    pepsin
  3.    vinculin
  4.    trypsin
Explanation:-
Answer: Option D. -> trypsin
Answer: (d).trypsin
Question 5. Which of the following techniques delivers the amino-acid sequence of a peptide?
  1.    Tandem MS
  2.    GC-MS
  3.    LC-MS
  4.    SDS-PAGE
Explanation:-
Answer: Option A. -> Tandem MS
Answer: (a).Tandem MS
Question 6. The procedure by which DNA gel is transferred to a membrane is termed as ___________________
  1.    blotting
  2.    centifugation
  3.    electrophoresis
  4.    paper chromatography
Explanation:-
Answer: Option A. -> blotting
Answer: (a).blotting
Question 7. In which of the following techniques, the DNA is labeled using complementary sequences and then analyzed using autoradiography?
  1.    Western blotting
  2.    Southern blotting
  3.    Isopycnic centrifugation
  4.    Spectrophotometry
Explanation:-
Answer: Option B. -> Southern blotting
Answer: (b).Southern blotting
Question 8. Which of the following molecules can be analyzed using a northern blot?
  1.    RNA
  2.    Carbohydrates
  3.    Proteins
  4.    DNA
Explanation:-
Answer: Option A. -> RNA
Answer: (a).RNA
Question 9. Which of the following is a commonly used label in blotting techniques?
  1.    vimentin
  2.    biotin
  3.    avidin
  4.    streptomycin
Explanation:-
Answer: Option B. -> biotin
Answer: (b).biotin
Question 10. Who developed the chemical techniques to synthesize polynucleotides?
  1.    Barbara McClintock
  2.    James Watson
  3.    Fredrick Sanger
  4.    H. Gobind Khorana
Explanation:-
Answer: Option D. -> H. Gobind Khorana
Answer: (d).H. Gobind Khorana
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