Chromatography(Analytical Instrumentation ) Questions and Answers

Question 1. Which of the following equations give the expression for plate number, N when ‘tR’ is the adjusted retention time and ‘Wb’ is the width at the base of the peak which is equal to 4 standard deviations.
  1.    16 tR²/Wb
  2.    4 tR²/Wb
  3.    (4tR/Wb)²
  4.    4 (tR/Wb)²
Explanation:-
Answer: Option C. -> (4tR/Wb)²
Answer: (c).(4tR/Wb)²
Question 2. It is more difficult to measure the width at half peak height than the base width in the detector output.
  1.    True
  2.    False
  3.    May be True or False
  4.    Can't say
Explanation:-
Answer: Option B. -> False
Answer: (b).False
Question 3. The solute, while moving in the column is always in equilibrium with both the stationary phase and the mobile phase.
  1.    True
  2.    False
  3.    May be True or False
  4.    Can't say
Explanation:-
Answer: Option A. -> True
Answer: (a).True
Question 4. For the separation of which of the following substances, Gas-solid chromatography is being used?
  1.    Thermally stable organic components
  2.    Volatile organic components
  3.    Thermally stable inorganic components
  4.    Low molecular weight gaseous species
Explanation:-
Answer: Option D. -> Low molecular weight gaseous species
Answer: (d).Low molecular weight gaseous species
Question 5. Which of the following is not a feature of carrier gas used in gas chromatography?
  1.    It must be chemically inert
  2.    It should be suitable for the detector employed
  3.    It should not be completely pure
  4.    It should be cheap
Explanation:-
Answer: Option C. -> It should not be completely pure
Answer: (c).It should not be completely pure
Question 6. Which of the following properties of molecular sieves make it ideal for exclusion mechanism of separation?
  1.    High thermal stability
  2.    Large inner surface area
  3.    Variable framework charge
  4.    Ability to distinguish materials on the basis of their size
Explanation:-
Answer: Option D. -> Ability to distinguish materials on the basis of their size
Answer: (d).Ability to distinguish materials on the basis of their size
Question 7. Which of the following is the disadvantage of reciprocating pump used in liquid chromatography?
  1.    Produces pulsed flow
  2.    Corrosive components
  3.    Does not have small hold-up value
  4.    Does not have moderate flow rate
Explanation:-
Answer: Option A. -> Produces pulsed flow
Answer: (a).Produces pulsed flow
Question 8. Which of the following is not a disadvantage of Pneumatic pumps used in liquid chromatography?
  1.    Pulsed output
  2.    Dependent on solvent viscosity
  3.    Dependent on back pressure
  4.    Inconvenient for solvent gradient elution
Explanation:-
Answer: Option A. -> Pulsed output
Answer: (a).Pulsed output
Question 9. Which of the following is not a desired characteristic of pulse dampers or flow smootheners used in liquid chromatography?
  1.    Easy mobile phase change over
  2.    Constant flow must be maintained
  3.    Should be effective at low system pressure
  4.    Maximal dead volume
Explanation:-
Answer: Option D. -> Maximal dead volume
Answer: (d).Maximal dead volume
Question 10. Which of the following will improve the efficiency of the separation process in liquid chromatography?
  1.    Increase in sample size, increase in column diameter
  2.    Reduction in sample size, increase in column diameter
  3.    Increase in sample size, reduction in column diameter
  4.    Reduction in sample size, reduction in column diameter
Explanation:-
Answer: Option D. -> Reduction in sample size, reduction in column diameter
Answer: (d).Reduction in sample size, reduction in column diameter
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