Enzyme Kinetics(Enzyme Technology ) Questions and Answers

Question 1. The covalent catalysis is aided by one of the following method?
  1.    General acid-base catalysis
  2.    Specific acid-base catalysis
  3.    Nucleophilic catalysis
  4.    Substrate collision theory
Explanation:-
Answer: Option C. -> Nucleophilic catalysis
Answer: (c).Nucleophilic catalysis
Question 2. ________________ involves substrates forming transient covalent bond with the residues present in the active site
  1.    Covalent catalysis
  2.    Specific acid-base catalysis
  3.    General acid-base catalysis
  4.    Lock and key model
Explanation:-
Answer: Option A. -> Covalent catalysis
Answer: (a).Covalent catalysis
Question 3. Daniel Koshland proposed a model for enzyme’s reaction mechanism in 1958 which is termed as __________
  1.    Induced fit model
  2.    Lock and key model
  3.    Henri kinetic model
  4.    Miachelis Menten kinetic model
Explanation:-
Answer: Option A. -> Induced fit model
Answer: (a).Induced fit model
Question 4. Which of this is not true for the active site of an enzyme?
  1.    Active site is constituted by 3 to 4 amino-acids in a big protein structure
  2.    Active site is mainly constituted by non-polar amino acids for catalysis to take place
  3.    Water is generally included in the site
  4.    Active site is a cleft formed by different amino-acids from different positions in an enzyme molecule
Explanation:-
Answer: Option C. -> Water is generally included in the site
Answer: (c).Water is generally included in the site
Question 5. Which is the first step involved in chymotrypsin mediated peptide bond hydrolysis?
  1.    Acylation
  2.    Specific acid-base catalysis
  3.    General acid-base catalysis
  4.    Deacylation
Explanation:-
Answer: Option D. -> Deacylation
Answer: (d).Deacylation
Question 6. Given an enzyme with Kₘ of 0.25mM, at what substrate concentration will the velocity of the enzyme reach 1/2 of the Vₘₐₓ? (Vₘₐₓ = 100 mmol/s)
  1.    0.5 mM
  2.    0.25 mM
  3.    0.75 mM
  4.    25 mM
Explanation:-
Answer: Option B. -> 0.25 mM
Answer: (b).0.25 mM
Question 7. An enzyme with a Kₘ of 5mM has a reaction rate of 100 mmol/min at substrate concentration of 0.25 mmol. What is the maximum reaction rate that this enzyme can achieve when its saturated with substrate?
  1.    2100
  2.    1500
  3.    1900
  4.    9000
Explanation:-
Answer: Option A. -> 2100
Answer: (a).2100
Question 8. What does the following graph represent?What Does The Following Graph Represent?
  1.    Miachelis Menten plot
  2.    Lineweaver Burk plot
  3.    Eadie-Hofstee plot
  4.    Hanes plot
Explanation:-
Answer: Option D. -> Hanes plot
Answer: (d).Hanes plot
Question 9. Which is the model proposed in the following diagram?Which Is The Model Proposed In The Following Diagram?
  1.    Fluid Mosaic Model
  2.    Lock and key model
  3.    Induced fit model
  4.    Miachelis Menten model
Explanation:-
Answer: Option C. -> Induced fit model
Answer: (c).Induced fit model
Question 10. What does the following plot represent?What Does The Following Plot Represent?
  1.    Miachelis Menten plot
  2.    Hanes plot
  3.    Lineweaver Burk plot
  4.    Eadie-Hofstee plot
Explanation:-
Answer: Option A. -> Miachelis Menten plot
Answer: (a).Miachelis Menten plot
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