Enzyme Kinetics Fundamentals(Enzyme Technology ) Questions and Answers

Question 1. In competitive inhibition, what happens to Vₘₐₓ and Kₘ if [I] = Kᵢ?
  1.    Lowers to 0.5 Vₘₐₓ and 0.5 Kₘ
  2.    Vₘₐₓ is unchanged and Kₘ increases 2Kₘ
  3.    Lowers to 0.5 Vₘₐₓ and Kₘ remains unchanged
  4.    Lowers to 0.67 Vₘₐₓ and Kₘ increases to 2Kₘ
Explanation:-
Answer: Option B. -> Vₘₐₓ is unchanged and Kₘ increases 2Kₘ
Answer: (b).Vₘₐₓ is unchanged and Kₘ increases 2Kₘ
Question 2. Which of these is correct for mixed inhibition?
  1.    Lowers to 0.5 Vₘₐₓ and 0.5 Kₘ
  2.    Vₘₐₓ is unchanged and Kₘ increases 2Kₘ
  3.    Lowers to 0.5 Vₘₐₓ and Kₘ remains unchanged
  4.    Lowers to 0.67 Vₘₐₓ and Kₘ increases to 2Kₘ
Explanation:-
Answer: Option D. -> Lowers to 0.67 Vₘₐₓ and Kₘ increases to 2Kₘ
Answer: (d).Lowers to 0.67 Vₘₐₓ and Kₘ increases to 2Kₘ
Question 3. If I = Kᵢ = Kᵢ‘, then what will happen to Vₘₐₓ and Kₘ when inhibitor acts non-competitively?
  1.    Lowers to 0.5 Vₘₐₓ and 0.5 Kₘ
  2.    Vₘₐₓ is unchanged and Kₘ increases 2Kₘ
  3.    Lowers to 0.5 Vₘₐₓ and Kₘ remains unchanged
  4.    Lowers to 0.67 Vₘₐₓ and Kₘ increases to 2Kₘ
Explanation:-
Answer: Option C. -> Lowers to 0.5 Vₘₐₓ and Kₘ remains unchanged
Answer: (c).Lowers to 0.5 Vₘₐₓ and Kₘ remains unchanged
Question 4. Mercury causes irreversible inhibition.
  1.    True
  2.    False
  3.    May be True or False
  4.    Can't say
Explanation:-
Answer: Option A. -> True
Answer: (a).True
Question 5. What happens to Vₘₐₓ and Kₘ in case of uncompetitive inhibition when I = Kᵢ‘?
  1.    Lowers to 0.5 Vₘₐₓ and 0.5 Kₘ
  2.    Vₘₐₓ is unchanged and Kₘ increases 2Kₘ
  3.    Lowers to 0.5 Vₘₐₓ and Kₘ remains unchanged
  4.    Lowers to 0.67 Vₘₐₓ and Kₘ increases to 2Kₘ
Explanation:-
Answer: Option A. -> Lowers to 0.5 Vₘₐₓ and 0.5 Kₘ
Answer: (a).Lowers to 0.5 Vₘₐₓ and 0.5 Kₘ
Question 6. Trypsin exhibits which kind of specificity?
  1.    Absolute specificity
  2.    Group specificity
  3.    Stereospecificity
  4.    Regiospecificity
Explanation:-
Answer: Option B. -> Group specificity
Answer: (b).Group specificity
Question 7. Which of the following rule was not given by the enzyme commission?
  1.    Assigned each enzyme a name
  2.    Assigned each enzyme a 4-digit code
  3.    Divided enzymes into 6 main groups
  4.    Mention of cofactors
Explanation:-
Answer: Option D. -> Mention of cofactors
Answer: (d).Mention of cofactors
Question 8. Enzymes that catalyze the transfer of atom or group between two molecules is known as __________
  1.    oxidoreductases
  2.    transferases
  3.    ligases
  4.    isomerases
Explanation:-
Answer: Option B. -> transferases
Answer: (b).transferases
Question 9. What kind of specificity does glucose oxidase exhibit?
  1.    Absolute specificity
  2.    Group specificity
  3.    Stereospecificity
  4.    Regiospecificity
Explanation:-
Answer: Option A. -> Absolute specificity
Answer: (a).Absolute specificity
Question 10. Pectinases belong to which class of enzyme?
  1.    Ligases
  2.    Hydrolases
  3.    Lyases
  4.    Transferases
Explanation:-
Answer: Option C. -> Lyases
Answer: (c).Lyases
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