Enzymes Purification(Enzyme Technology ) Questions and Answers

Question 1. Which of the following is not a step involved in purification?
  1.    Precipitation
  2.    Dialysis or ultrafiltration
  3.    Chromatographic techniques
  4.    Freezing technique
Explanation:-
Answer: Option D. -> Freezing technique
Answer: (d).Freezing technique
Question 2. Which of the following is a disadvantage of organic solvent fractionation?
  1.    Faster than column techniques
  2.    Denaturation of proteins
  3.    Low density than salt solutions
  4.    Short centrifugation times
Explanation:-
Answer: Option B. -> Denaturation of proteins
Answer: (b).Denaturation of proteins
Question 3. His-Tag allows the purification to be done in fewer steps.
  1.    True
  2.    False
  3.    May be True or False
  4.    Can't say
Explanation:-
Answer: Option A. -> True
Answer: (a).True
Question 4. Purifications of purified proteins involved in small scale lab productions meant mainly for research purposes are referred to as _____________
  1.    preparative purifications
  2.    chromatographic techniques
  3.    analytical purifications
  4.    dialysis techniques
Explanation:-
Answer: Option C. -> analytical purifications
Answer: (c).analytical purifications
Question 5. Insulin is purified under ___________ purifications.
  1.    analytical
  2.    chromatography
  3.    preparative
  4.    ultrafiltration
Explanation:-
Answer: Option C. -> preparative
Answer: (c).preparative
Question 6. In affinity chromatography, the matrix should be chemically and mechanically stable.
  1.    True
  2.    False
  3.    May be True or False
  4.    Can't say
Explanation:-
Answer: Option A. -> True
Answer: (a).True
Question 7. Which among these is not a way of non-specific elution?
  1.    Solvent change
  2.    pH change
  3.    Reversible denaturation
  4.    Competition to suitable complementary protein
Explanation:-
Answer: Option D. -> Competition to suitable complementary protein
Answer: (d).Competition to suitable complementary protein
Question 8. The matrix selected during affinity chromatography must possess certain chemical groups.
  1.    True
  2.    False
  3.    May be True or False
  4.    Can't say
Explanation:-
Answer: Option A. -> True
Answer: (a).True
Question 9. The first step in preparation of affinity chromatography column is _____________
  1.    ligand attachment to matrix
  2.    coupling of aromatic amines to matrix
  3.    activation process
  4.    precipitation
Explanation:-
Answer: Option C. -> activation process
Answer: (c).activation process
Question 10. __________ refers to eluting a compound by creating a competition between the bounded enzymes with its suitable complimentary protein in solution.
  1.    Specific elution
  2.    Nonspecific elution
  3.    Filtration
  4.    Dialysis
Explanation:-
Answer: Option A. -> Specific elution
Answer: (a).Specific elution
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