Cloning Vectors(Biotechnology ) Questions and Answers

Question 1. Plasmid incompatibility is
  1.    inability of a plasmid to grow in the host
  2.    inability of two different plasmids to coexist in the same host cell in the absence of selection pressure.
  3.    both (a) and (b)
  4.    none of the above
Explanation:-
Answer: Option B. -> inability of two different plasmids to coexist in the same host cell in the absence of selection pressure.
Answer: (b).inability of two different plasmids to coexist in the same host cell in the absence of selection pressure.
Question 2. P1 cloning vector is the example of
  1.    plasmid
  2.    cosmid
  3.    bacteriophage
  4.    phagemid
Explanation:-
Answer: Option C. -> bacteriophage
Answer: (c).bacteriophage
Question 3. P1 cloning vector allow cloning of DNA of the length of
  1.    100 kbp
  2.    50 kbp
  3.    20 kbp
  4.    10 kbp
Explanation:-
Answer: Option A. -> 100 kbp
Answer: (a).100 kbp
Question 4. Charon 34 and Charon 35 can clone DNA upto
  1.    1-2 kb
  2.    6-7 kb
  3.    9-20 kb
  4.    30-35 kb
Explanation:-
Answer: Option C. -> 9-20 kb
Answer: (c).9-20 kb
Question 5. Charon vectors are different from EMBL vectors because
  1.    they have more extensive range of restriction targets with in their polylinkers.
  2.    physical separation of lambda arm from central fragment is required
  3.    both (a) and (b)
  4.    physical separation of lambda arm from central fragment is not required
Explanation:-
Answer: Option C. -> both (a) and (b)
Answer: (c).both (a) and (b)
Question 6. Cosmids lack
  1.    genes coding for viral proteins
  2.    origin of replication
  3.    marker genes coding for replication
  4.    cleavage site for the insertion of foreign DNA
Explanation:-
Answer: Option A. -> genes coding for viral proteins
Answer: (a).genes coding for viral proteins
Question 7. Charon 34 and Charon 35 are the examples of
  1.    plasmid vector
  2.    cosmid vector
  3.    phage vector
  4.    phagemid vector
Explanation:-
Answer: Option C. -> phage vector
Answer: (c).phage vector
Question 8. EMBL 3 and EMBL 4 are replacement vectors, which can clone DNA up to
  1.    6-7 kb
  2.    15-25 kb
  3.    40-44 kb
  4.    1-2 kb
Explanation:-
Answer: Option B. -> 15-25 kb
Answer: (b).15-25 kb
Question 9. Cosmid vectors are used for
  1.    cloning small fragments of DNA
  2.    cloning large fragments of DNA
  3.    cloning prokaryotic DNA only
  4.    cloning eukaryotic DNA only
Explanation:-
Answer: Option B. -> cloning large fragments of DNA
Answer: (b).cloning large fragments of DNA
Question 10. Size of the DNA that can be packaged into a λ phage is
  1.    50 kb
  2.    35-53 kb
  3.    40-50 kb
  4.    any size
Explanation:-
Answer: Option B. -> 35-53 kb
Answer: (b).35-53 kb
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