Enzyme Extraction And Isolation(Enzyme Technology ) Questions and Answers

Question 1. Which of the following is the best method for isolating enzymes from cell free extract?
  1.    pH treatment
  2.    Temperature treatment
  3.    Chemical treatment
  4.    Osmotic shock
Explanation:-
Answer: Option C. -> Chemical treatment
Answer: (c).Chemical treatment
Question 2. Which of the following chemical is not used to isolate enzymes from cell free extract?
  1.    Polyethyleneimine
  2.    Polyvinylpyrrolidone
  3.    EDTA
  4.    Magnesium chloride
Explanation:-
Answer: Option C. -> EDTA
Answer: (c).EDTA
Question 3. Which of the following methods cannot be used to determine the molecular weight of enzymes?
  1.    Mass spectrometry
  2.    Gel filtration
  3.    Biosensors
  4.    Ultracentrifugation
Explanation:-
Answer: Option D. -> Ultracentrifugation
Answer: (d).Ultracentrifugation
Question 4. __________ is a compound which is frequently used for DNA removal.
  1.    1.4% buffered sucrose
  2.    Protamine sulphate
  3.    Sodium hydroxide
  4.    Lysozyme
Explanation:-
Answer: Option B. -> Protamine sulphate
Answer: (b).Protamine sulphate
Question 5. Cetyl trimethyl ammonium bromide is a cationic detergent.
  1.    True
  2.    False
  3.    May be True or False
  4.    Can't say
Explanation:-
Answer: Option A. -> True
Answer: (a).True
Question 6. Gram positive bacteria are highly susceptible to lysozyme treatment.
  1.    True
  2.    False
  3.    May be True or False
  4.    Can't say
Explanation:-
Answer: Option A. -> True
Answer: (a).True
Question 7. In ________ treatment, produced basic radicals mediate a nucleophilic attack on compounds of cell wall on membrane leading to destabilization and fragmentation of membrane followed by release of enzymes.
  1.    EDTA
  2.    Osmotic shock
  3.    Alkali
  4.    Detergent
Explanation:-
Answer: Option C. -> Alkali
Answer: (c).Alkali
Question 8. Which of the following is not a technique for the extraction of enzymes by chemical methods?
  1.    Alkali treatment
  2.    Osmotic shock
  3.    Detergent treatment
  4.    Homogenization
Explanation:-
Answer: Option D. -> Homogenization
Answer: (d).Homogenization
Question 9. In ___________ treatment, certain molecules interact through ionic or Vander wall forces which leads to solubilization of phospholipids and lipoprotein which in turn, increases membrane permeability directing to cell lysis.
  1.    Lysozyme
  2.    EDTA
  3.    Detergent
  4.    NaoH
Explanation:-
Answer: Option C. -> Detergent
Answer: (c).Detergent
Question 10. ____________ is an enzyme, which is highly produced by egg white and lachrymal glands.
  1.    Amylases
  2.    Lysozyme
  3.    Invertase
  4.    Protease
Explanation:-
Answer: Option B. -> Lysozyme
Answer: (b).Lysozyme
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