Immobilized Enzymes And Their Uses(Enzyme Technology ) Questions and Answers

Question 1. ____________ is produced with the help of immobilized penicillin -G-amidase by adsorption to DEAE -cellulose in a packed bed column.
  1.    Penicillin
  2.    Ampicillin
  3.    L-aminoacids
  4.    L-aspartic acid
Explanation:-
Answer: Option B. -> Ampicillin
Answer: (b).Ampicillin
Question 2. What does ‘X’ represent in the following reaction?
6-aminopenicillanic acid + D-phenylglyicine methyl ester \(_{\overrightarrow{Penicillin \,amidases}}\) X + methanol
  1.    Phenylacetic acid
  2.    Phenoxyacetic acid
  3.    Ampicillin
  4.    L-aspartic acid
Explanation:-
Answer: Option C. -> Ampicillin
Answer: (c).Ampicillin
Question 3. What does ‘Z’ represent in the following reaction?
Phenoxymethyl-penicillin + water \(\underset{Z}{\rightarrow}\) 6-aminopenicillianic acid + Phenoxyacetic acid
  1.    Aspartases
  2.    Hydantoinase
  3.    Lactases
  4.    Penicillin amidases
Explanation:-
Answer: Option D. -> Penicillin amidases
Answer: (d).Penicillin amidases
Question 4. Copper acts as a good catalyst in production of acrylamide.
  1.    True
  2.    False
  3.    May be True or False
  4.    Can't say
Explanation:-
Answer: Option B. -> False
Answer: (b).False
Question 5. What does ‘Y’ represent in the following reaction?
Benzyl-penicillin + water \(_{\overrightarrow{Penicillin \,amidases}}\) Phenylacetic acid + Y
  1.    6-aminopenicillianic acid
  2.    Phenoxyacetic acid
  3.    Ampicillin
  4.    L-aspartic acid
Explanation:-
Answer: Option A. -> 6-aminopenicillianic acid
Answer: (a).6-aminopenicillianic acid
Question 6. What does ‘X’ represent in the following reaction?
Acrylonitrile + water \(\underset{Cu}{\rightarrow}\) X
  1.    L-aspartic acid
  2.    Ampicillin
  3.    Acrylamide
  4.    Penicillin
Explanation:-
Answer: Option C. -> Acrylamide
Answer: (c).Acrylamide
Question 7. Immobilized ____________ is used in the production of acrylamide.
  1.    aspartase
  2.    raffinase
  3.    nitrilase
  4.    penicillin amidases
Explanation:-
Answer: Option C. -> nitrilase
Answer: (c).nitrilase
Question 8. Nitrilase is obtained from __________
  1.    Rhodococcus
  2.    Mortierella vinacea
  3.    Escherichia coli
  4.    Yeast
Explanation:-
Answer: Option A. -> Rhodococcus
Answer: (a).Rhodococcus
Question 9. Immobilized ___________ is prepared by entrapping the intact Rhodococcus cells in a cross-linked polyacrylamide/dimethylaminoethylmethacrylate gel.
  1.    Raffinase
  2.    Nitrilase
  3.    Penicillin amidases
  4.    Aminoacyclase
Explanation:-
Answer: Option B. -> Nitrilase
Answer: (b).Nitrilase
Question 10. Immobilized Rhodococcus is used at 10˚C and pH 8.0-8.5 in a semi-batchwise process with low acrylonitrile concentration to produce __________
  1.    L-aspartic acid
  2.    Ampicillin
  3.    Acrylamide
  4.    Aminoacids
Explanation:-
Answer: Option C. -> Acrylamide
Answer: (c).Acrylamide
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